|
| Status |
Public on Nov 10, 2022 |
| Title |
WT-sham-B636 |
| Sample type |
SRA |
| |
|
| Source name |
kidney
|
| Organism |
Mus musculus |
| Characteristics |
tissue: kidney
cell type: renal cortex cells
genotype: WT
treatment: control
time: Day 28
disease state: control
|
| Treatment protocol |
The sham mice were administered the same amount of PBS. After 28 days, the mice were euthanized and the kidney tissue samples were harvested for histopathological analysis.
Aristolochic acid nephropathy was induced by a one-time intraperitoneal injection of aristolochic acid (5 mg/kg body weight, A5512, Sigma-Aldrich) in PBS. After 28 days, the mice were euthanized and the kidney tissue samples were harvested for histopathological analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
MJzol animal RNA Extraction Kit(MagBeads)
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Sequenced reads were trimmed for adaptor sequence and masked for low-complexity or low-quality sequence by using Seqtk.
The sample was mapped using Hisat2（version:2.0.4)
The quantification of transcripts expressed for each sequenced samples was performed using Stringtie（version:1.3.0）
Differentially expressed genes were identified using edgeR
Assembly: mm10
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample
|
| |
|
| Submission date |
Nov 03, 2022 |
| Last update date |
Nov 11, 2022 |
| Contact name |
Zhang Yang |
| E-mail(s) |
201915118@mail.sdu.edu.cn
|
| Phone |
17863129058
|
| Organization name |
Shandong University
|
| Department |
Department of Pharmacology
|
| Street address |
44 Wenhua West Road
|
| City |
Jinan |
| ZIP/Postal code |
250012 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (1) |
| GSE217176 |
HDAC9-mediated Epithelial Cell Cycle Arrest in G2/M Contributes to Kidney Fibrosis |
|
| Relations |
| BioSample |
SAMN31582197 |
| SRA |
SRX18154662 |
