|
Status |
Public on Feb 27, 2024 |
Title |
Bulk RNAseq NGN2 AroLITE Rep 3 |
Sample type |
SRA |
|
|
Source name |
ZIP13K2
|
Organism |
Homo sapiens |
Characteristics |
cell type: ZIP13K2 age: 5d
|
Extracted molecule |
polyA RNA |
Extraction protocol |
ZIP13K2 induced neurons were harvested following the Direct-zolTM RNA MicroPrep Kit (Zymo Research) standard protocol 1 μg of RNA of each sample was used as input for library preparation using the KAPA Stranded mRNA-Seq Kit (Roche) following the manufacturer’s instructions. Unique Dual-Indexed Set-B (UDI; KAPA biosystems) adapters were ligated and the library was amplified for 8 cycles Paired-end 100 with 50 million fragments per library
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
Bulk RNA-seq
|
Data processing |
Reads were mapped to a genome using STAR aligner. Count read tables were generated by the same program genome_build: hg38
|
|
|
Submission date |
Nov 04, 2022 |
Last update date |
Feb 27, 2024 |
Contact name |
Alexandre P Magalhaes |
E-mail(s) |
magalhae@molgen.mpg.de
|
Organization name |
Max Planck Institute for Molecular Genetics
|
Street address |
Ihnestrasse 63-73
|
City |
Berlin |
ZIP/Postal code |
14194 |
Country |
Germany |
|
|
Platform ID |
GPL24676 |
Series (1) |
GSE201655 |
An activity-specificity trade-off encoded in human transcription factors |
|
Relations |
BioSample |
SAMN31610427 |
SRA |
SRX18173741 |