Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Other
Summary
Transcription factors play essential roles in cell type specification during differentiation, and small sets of transcription factors can reprogram cell identity. Therefore, we tested the impact of increasing periodicity of aromatic residues on the ability of transcription factors to reprogram cells.
Overall design
We performed multiple RNAseq, scRNAseq, TTSlamSeq, and ChIPseq of the cells expressing the WT, "AroLITE" and "AroPERFECT" of CEBPa, HOXD4, NGN2, and MYOD1 proteins after transgene induction to measure differentiation of specific cell lines.
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