|
| Status |
Public on Feb 27, 2024 |
| Title |
Bulk RNAseq C2C12 WT Rep 1 |
| Sample type |
SRA |
| |
|
| Source name |
C2C12
|
| Organism |
Mus musculus |
| Characteristics |
cell type: C2C12
age: 4d
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
C2C12 induced cells were harvested following the Direct-zolTM RNA MicroPrep Kit (Zymo Research) standard protocol
1 μg of RNA of each sample was used as input for library preparation using the KAPA Stranded mRNA-Seq Kit (Roche) following the manufacturer’s instructions. Unique Dual-Indexed Set-B (UDI; KAPA biosystems) adapters were ligated and the library was amplified for 8 cycles
Paired-end 100 with 50 million fragments per library
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Bulk RNA-seq
|
| Data processing |
Reads were mapped to a genome using STAR aligner. Count read tables were generated by the same program
genome_build: mm39
|
| |
|
| Submission date |
Nov 04, 2022 |
| Last update date |
Feb 27, 2024 |
| Contact name |
Alexandre P Magalhaes |
| E-mail(s) |
magalhae@molgen.mpg.de
|
| Organization name |
Max Planck Institute for Molecular Genetics
|
| Street address |
Ihnestrasse 63-73
|
| City |
Berlin |
| ZIP/Postal code |
14194 |
| Country |
Germany |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE201655 |
Suboptimization of human transcription factors |
|
| Relations |
| BioSample |
SAMN31610423 |
| SRA |
SRX18173745 |
