|
| Status |
Public on Nov 15, 2022 |
| Title |
TCR-seq_TD3 |
| Sample type |
SRA |
| |
|
| Source name |
Sorted T cells from Braf/Pten induciable melanoma tumor with disconued T cell depletion
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: Sorted CD45+ CD3+ CD8+ CD44+ tumor-infiltrating T cells
tumor type: Braf/Pten inducible melanoma tumor
treatment: T cell depletion
|
| Treatment protocol |
Tumor bearing Braf/PTEN mice, two weeks after tumor induction, were treated with a gradually decreased dosage of anti-CD4 and anti-CD8 antibodies.
|
| Growth protocol |
Braf/Pten mice were treated with 4-hydroxytamoxifen (4-HT) on the skin surface to induce tumor formation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TILs were lysis by Lysis/Binding Buffer for mRNA purification.
Murine TCR beta-chain specific primer was used for amplification
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
NextSeq 1000 |
| |
|
| Data processing |
TCR repertoire analysis and clonotype identification was done by MiXCR
The diversity estimation was quantified by “immunarch” package in R
Assembly: Mouse TCR library
Library strategy: TCR_seq
|
| |
|
| Submission date |
Nov 12, 2022 |
| Last update date |
Nov 16, 2022 |
| Contact name |
Ping-Chih Ho |
| Organization name |
Ludwig Cancer Institute, UNIL
|
| Street address |
Ch. des Boveresses 155
|
| City |
Epalinges |
| ZIP/Postal code |
1066 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL32159 |
| Series (2) |
| GSE217482 |
Immunoediting instructs tumor metabolic reprogramming to support immune evasion |
| GSE217861 |
Immunoediting instructs tumor metabolic reprogramming to support immune evasion [TCR-seq] |
|
| Relations |
| BioSample |
SAMN31701416 |
| SRA |
SRX18243592 |
