|
| Status |
Public on Jan 15, 2024 |
| Title |
siSOX9 Rep 1 |
| Sample type |
SRA |
| |
|
| Source name |
A549
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
cell type: Lung adenocarcinoma
treatment: transfection siSOX9
|
| Growth protocol |
A549 Cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) in humidified atmosphere with 5% CO2 at 37C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from cells using TRIzol (Thermo Fisher Scientific).
NEBnext Ultra ii Stranded mRNA (New England Biolabs)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
De-multiplexed read sequences were aligned to the reference genome using STAR_2.5.0b.
Assembly and differential expression was estimated using Cufflinks 2.2.1 through bioinformatics apps available on Illumina Sequence Hub.
Assembly: hg19/GRCh37
Supplementary files format and content: Differentially expressed genes data tables
|
| |
|
| Submission date |
Nov 13, 2022 |
| Last update date |
Jan 15, 2024 |
| Contact name |
Bettina Maria Fuglerud |
| E-mail(s) |
bfuglerud@bccrc.ca
|
| Organization name |
BC Cancer
|
| Street address |
675 W 10th Ave
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE217880 |
Transcriptome sequencing of A549 cells |
|
| Relations |
| BioSample |
SAMN31707170 |
| SRA |
SRX18246424 |
