|
Status |
Public on Jan 15, 2024 |
Title |
siSOX9-TGFB Rep 3 |
Sample type |
SRA |
|
|
Source name |
A549
|
Organism |
Homo sapiens |
Characteristics |
cell line: A549 cell type: Lung adenocarcinoma treatment: transfection siSOX9 and TGFB1 treatment
|
Growth protocol |
A549 Cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) in humidified atmosphere with 5% CO2 at 37C
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted from cells using TRIzol (Thermo Fisher Scientific). NEBnext Ultra ii Stranded mRNA (New England Biolabs)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
De-multiplexed read sequences were aligned to the reference genome using STAR_2.5.0b. Assembly and differential expression was estimated using Cufflinks 2.2.1 through bioinformatics apps available on Illumina Sequence Hub. Assembly: hg19/GRCh37 Supplementary files format and content: Differentially expressed genes data tables
|
|
|
Submission date |
Nov 13, 2022 |
Last update date |
Jan 15, 2024 |
Contact name |
Bettina Maria Fuglerud |
E-mail(s) |
bfuglerud@bccrc.ca
|
Organization name |
BC Cancer
|
Street address |
675 W 10th Ave
|
City |
Vancouver |
State/province |
BC |
ZIP/Postal code |
V5Z 1L3 |
Country |
Canada |
|
|
Platform ID |
GPL16791 |
Series (1) |
GSE217880 |
Transcriptome sequencing of A549 cells |
|
Relations |
BioSample |
SAMN31707165 |
SRA |
SRX18246429 |