|
| Status |
Public on May 15, 2023 |
| Title |
Spatial_10x_PCW4_20220122_slice4 |
| Sample type |
SRA |
| |
|
| Source name |
embryo
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: embryo
age: PCW4
region: embryo
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Human embryo samples were collected in ice-cold artificial cerebrospinal fluid (ACSF), single cell was selected by FACS
Chromium™ Single Cell 3’ Reagent Kits v2
Visium Spatial Gene Expression Slide & Reagent Kit
Chromium Single Cell 3ʹ Reagent Kits v3
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Illumina Casava1.8 software used for basecalling
Reads were aligned to hg19 human genome and mm10 mosue genome by Cellranger(v2.0.1 and v3.0.2) and Space Ranger (version 1.0.0)
Filtered count matrix was merged by Scanpy Package(v1.4.2)
Assembly: hg19, mm10
Supplementary files format and content: Filtered count matrix was formatted by mtx, and barcode and gene information was with tsv format
|
| |
|
| Submission date |
Nov 17, 2022 |
| Last update date |
May 15, 2023 |
| Contact name |
Zeyuan Liu |
| Organization name |
Institute of Biophysics,Chinese Academy of Sciences
|
| Street address |
15 Datun Road, Chaoyang District, Beijing, 100101, China
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL20301 |
| Series (1) |
| GSE155121 |
The Single-cell and spatial transcriptional landscape of human development |
|
| Relations |
| BioSample |
SAMN31774374 |
| SRA |
SRX18297604 |
