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Sample GSM674072 Query DataSets for GSM674072
Status Public on Feb 20, 2012
Title IRT5913_T00245025
Sample type RNA
 
Source name Exfoliated colonocytes
Organism Rattus norvegicus
Characteristics strain: Sprague Dawley
diet: FO/P
injection: AOM
time: 7 wk after AOM injection
Treatment protocol Rats consumed diets containing FO/P or CO/C and were injected with azoxymethane (AOM, 2x, 15 mg/kg BW, s.c.).
Growth protocol Rats were housed individually in a temperature and humidity controlled animal facility with a 12 h light/dark cycle.
Extracted molecule total RNA
Extraction protocol To enrich the level of eukaryotic mRNA in the fecal samples, poly (A)+ RNA was isolated from total RNA using oligo dT cellulose micro spin columns and the mTRAP Maxi kit.
Label biotin
Label protocol Ambion MessageAmp II aRNA Amplification and Biotin Enhanced kits: Manual Labelled cRNA Target preparation following manufacturer's instructions http://www.ambion.com/techlib/prot/fm_1751.pdf and http://www.ambion.com/techlib/prot/fm_1791.pdf
 
Hybridization protocol Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf
Scan protocol Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/application_notes/63005079.pdf
Description T00245025_2011-2-8_IRT 5913 8WK
Data processing Median normalized values derived from Codelink Expression Analysis Version 5.0
 
Submission date Feb 12, 2011
Last update date Feb 20, 2012
Contact name Youngmi Cho
E-mail(s) ym473@tamu.edu
Phone 979-845-4887
Organization name Texas A&M University
Department Faculty of Nutrition
Street address 2253 TAMU
City College Station
State/province TX
ZIP/Postal code 77840
Country USA
 
Platform ID GPL2896
Series (1)
GSE27266 Fish oil/pectin temporally alters gene expression profiles in exfoliated rat colonocytes throughout oncogenesis

Data table header descriptions
ID_REF
VALUE Median normalized values (signals) derived from Codelink Expression Analysis Version 5.0

Data table
ID_REF VALUE
1001 539.9801
1002 0.7852
1004 6.227
1005 29.7352
1006 19.5559
1007 534.0726
1008 472.379
1009 11.3443
1010 0.2675
1011 1.6874
1012 2.6681
1013 71.2632
1014 476.8276
1015 483.612
1016 2.1526
1017 0.4794
1018 0.4775
1019 1.3434
1020 21.4825
1021 481.5533

Total number of rows: 35974

Table truncated, full table size 483 Kbytes.




Supplementary file Size Download File type/resource
GSM674072.TXT.gz 1.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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