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Sample GSM674101 Query DataSets for GSM674101
Status Public on Feb 20, 2012
Title IRT5913_T00245585
Sample type RNA
 
Source name Exfoliated colonocytes
Organism Rattus norvegicus
Characteristics strain: Sprague Dawley
diet: FO/P
injection: AOM
time: 28 wk after AOM injection
Treatment protocol Rats consumed diets containing FO/P or CO/C and were injected with azoxymethane (AOM, 2x, 15 mg/kg BW, s.c.).
Growth protocol Rats were housed individually in a temperature and humidity controlled animal facility with a 12 h light/dark cycle.
Extracted molecule total RNA
Extraction protocol To enrich the level of eukaryotic mRNA in the fecal samples, poly (A)+ RNA was isolated from total RNA using oligo dT cellulose micro spin columns and the mTRAP Maxi kit.
Label biotin
Label protocol Ambion MessageAmp II aRNA Amplification and Biotin Enhanced kits: Manual Labelled cRNA Target preparation following manufacturer's instructions http://www.ambion.com/techlib/prot/fm_1751.pdf and http://www.ambion.com/techlib/prot/fm_1791.pdf
 
Hybridization protocol Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/pdf/codelink_bioarray_processing_protocol.pdf
Scan protocol Codelink Gene Expression System: Single-Assay Bioarray Hybridization and Detection according to manufacturer's instructions http://www.appliedmicroarrays.com/application_notes/63005079.pdf
Description T00245585_2011-2-2_IRT 5913 28WK
Data processing Median normalized values derived from Codelink Expression Analysis Version 5.0
 
Submission date Feb 12, 2011
Last update date Feb 20, 2012
Contact name Youngmi Cho
E-mail(s) ym473@tamu.edu
Phone 979-845-4887
Organization name Texas A&M University
Department Faculty of Nutrition
Street address 2253 TAMU
City College Station
State/province TX
ZIP/Postal code 77840
Country USA
 
Platform ID GPL2896
Series (1)
GSE27266 Fish oil/pectin temporally alters gene expression profiles in exfoliated rat colonocytes throughout oncogenesis

Data table header descriptions
ID_REF
VALUE Median normalized values (signals) derived from Codelink Expression Analysis Version 5.0

Data table
ID_REF VALUE
1001 268.7577
1002 0.3082
1004 2.918
1005 40.8582
1006 38.2981
1007 267.9878
1008 295.7501
1009 15.0088
1010 0.594
1011 1.2074
1012 2.1487
1013 21.5049
1014 290.0557
1015 281.0417
1016 0.3726
1017 1.6315
1018 0.748
1019 0.6744
1020 26.1624
1021 264.1863

Total number of rows: 35974

Table truncated, full table size 484 Kbytes.




Supplementary file Size Download File type/resource
GSM674101.TXT.gz 1.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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