|
| Status |
Public on Mar 27, 2024 |
| Title |
NK_atRA_7days_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
splenic NK cells, atRA and IL-2, 7days
|
| Organism |
Mus musculus |
| Characteristics |
age: adult
Sex: female+male
genotype: Rag 2 ko
strain: BL6
tissue: spleen
cell type: sorted NK cells
|
| Treatment protocol |
NK cells were treated with 1uM all-trans retinoic acid or DMSO 0.01% v/v (as solvent control) for 7 days
|
| Growth protocol |
NK cells sorted as CD45+CD3-NK1.1+NKp46+TRAIL-CD200R- were cultured in RPMI-1640 medium supplemented with 10% FCS, 2 mM glutamine, 1% non-essential amino acids, 1 mM sodium pyruvate, 100 U/ml Penicillin/Streptomycin, 50 µM β-mercaptoethanol and 1700 U/ml recombinant human IL-2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using RNeasy® Mini Kit (Qiagen). DNA was removed using TURBO DNA‐free™ kit (ThermoFisher)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Gene expression data after treatment
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 15, from SAS. Gene expression profiling was performed using arrays of Mouse Clariom D from Thermo Fischer Scientific. A Custom CDF Version 22 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
|
| |
|
| Submission date |
Nov 22, 2022 |
| Last update date |
Mar 27, 2024 |
| Contact name |
Carolina Delatorre |
| E-mail(s) |
carolina.delatorre@medma.uni-heidelberg.de
|
| Organization name |
University Heidelberg
|
| Street address |
Theodor-Kutzer-Ufer 1-3
|
| City |
Mannheim |
| ZIP/Postal code |
68167 |
| Country |
Germany |
| |
|
| Platform ID |
GPL32874 |
| Series (1) |
| GSE218594 |
Vitamin A-treated NK cells reduce IFN-γ production and support regulatory T cell differentiation |
|
