|
Status |
Public on Nov 08, 2023 |
Title |
P1AM25 [Subarray 30] |
Sample type |
protein |
|
|
Source name |
monoclonal antibody
|
Organism |
Homo sapiens |
Characteristics |
antibody: monoclonal antibody
|
Treatment protocol |
Diluted to 5 µg/mL
|
Growth protocol |
N/A
|
Extracted molecule |
protein |
Extraction protocol |
MAb expressed in HEK293 cell
|
Label |
SureLight®P3 Cy5
|
Label protocol |
SureLight®P3 Cy5
|
|
|
Hybridization protocol |
Arrays were blocked with 3% BSA overnight, and diluted mAb was incubated on the array for 4 hours at 37 C and gentle agitation (100 RPM). Slides were then washed with PBS contain 0.05% Tween (PBST). After washing, bound antibodies were detected by goat anti-human biotin-labeled IgG (Jackson Immunoresearch), followed by a Streptavidin probe tagged with SureLight®P3 Cy5 (Cayman Chemicals). Slides were then washed with PBST, spun dry in a centrifuge, and scanned.
|
Scan protocol |
The GenePix 4000 Microarray scanner system (Molecular Devices, CA) was used for scanning.
|
Description |
IgG
|
Data processing |
We analyzed images using GenePix Pro 7.3.0.0 to measure median pixel intensity (MPI) and neighboring background pixel intensity (BPI) of individual spots. The median fluorescence intensity (MFI), representing AM-epitope specific mAb reactivity, was calculated using the MPI minus the BPI and averaged from the triplicate spots. average (background-subtracted median pixel intensity for technical triplicates)
|
|
|
Submission date |
Nov 28, 2022 |
Last update date |
Nov 08, 2023 |
Contact name |
Jacqueline M. Achkar |
Organization name |
Albert Einstein College of Medicine
|
Street address |
1300 Morris Park Avenue
|
City |
Bronx |
ZIP/Postal code |
10461 |
Country |
USA |
|
|
Platform ID |
GPL32887 |
Series (1) |
GSE218873 |
Features and protective efficacy of human monoclonal antibodies targeting M. tuberculosis arabinomannan |
|