|
| Status |
Public on Feb 22, 2011 |
| Title |
WT_6.6mM |
| Sample type |
RNA |
| |
|
| Source name |
Bacteroides fragilis strain YCH46
|
| Organism |
Bacteroides fragilis YCH46 |
| Characteristics |
strain: YCH46
genotype/variation: Wild-type
culture condition: Pi-excess (6.6mM Pi) condition
|
| Growth protocol |
Bacteroides fragilis strains were grown to mid-log phase (O.D.600nm 0.4-0.6) in defined minimal media (Varel, V.H et al. Appl. Microbiol.28(2):251-257, 1974.) supplemented with 6.6 mM or 0.0066 mM of KH2PO4.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using hot-phenol method.
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| Description |
Wild 6.6
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis), quantile normalization, and background correction as implemented in the ArrayStar software (DNASTAR)
|
| |
|
| Submission date |
Feb 22, 2011 |
| Last update date |
Feb 22, 2011 |
| Contact name |
Haruyuki Nakayama-Imaohji |
| E-mail(s) |
nakayama@basic.med.tokushima-u.ac.jp
|
| Organization name |
Tokushima University
|
| Street address |
3-18-15 kuramoto-cho
|
| City |
tokushima |
| ZIP/Postal code |
770-8503 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13213 |
| Series (1) |
| GSE27439 |
Identification of the regulatory circuit of PhoB in Bacteroides fragilis strain YCH46 |
|
