|
| Status |
Public on Jul 09, 2023 |
| Title |
obe1-2_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Arabidopsis seedlings
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
cell type: Arabidopsis seedlings
ecotype: Col-0
genotype: obe1-/- obe2-/-
time: 11-day-old
|
| Treatment protocol |
none
|
| Growth protocol |
Arabidopsis seedlings were grown on MS (Murashige and Skoog) medium plates for 11 days, under a 16 h light/8 h dark photoperiod at 22°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 11-day-old Arabidopsis plants with TRIzol reagent (Invitrogen).
Sequencing libraries were built for RNA-seq (Illumina Novaseq 6000; 150-bp pair-end sequencing).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Basecalling performed using bcl2fastq.
The clean reads were aligned to the Arabidopsis genome (TAIR10) using hisat2.
The expression of genes were obtained with the R package edgeR.
Assembly: TAIR10
Supplementary files format and content: Tab-delimited text file include normalized reads for each sample.
|
| |
|
| Submission date |
Dec 23, 2022 |
| Last update date |
Jul 09, 2023 |
| Contact name |
xinjian He |
| E-mail(s) |
hexinjian@nibs.ac.cn
|
| Organization name |
National Institute of Biological Sciences
|
| Lab |
hexinjian
|
| Street address |
No. 7, zhongguancun life science park road, changping district
|
| City |
Beijing |
| ZIP/Postal code |
102206 |
| Country |
China |
| |
|
| Platform ID |
GPL26208 |
| Series (2) |
| GSE221658 |
A group of WRKY transcription factors form a protein complex with PHD-containing proteins to repress the transcription of stress responsive genes [RNA-Seq] |
| GSE221660 |
A group of WRKY transcription factors form a protein complex with PHD-containing proteins to repress the transcription of stress responsive genes |
|
| Relations |
| BioSample |
SAMN32386528 |
| SRA |
SRX18837019 |
