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Sample GSM698133 Query DataSets for GSM698133
Status Public on Apr 01, 2012
Title SHAM (Cy5) x PMCAO (Cy3) 6h
Sample type RNA
 
Channel 1
Source name Treatment Cy3
Organism Mus musculus
Characteristics strain: C57BL/6J
tissue: brain
treatment: permanent middle cerebral artery occlusion
time: 6h
Treatment protocol PMCAO control 6 h
Growth protocol Forty male mice (8 to 12-weeks-old, 25 ~ 35 g body weight) were housed at the Animal Institution in Showa University in acrylic cages (8 mice / cage) at 23ºC, and maintained with a standard 12 h light/dark cycle with optimum humidity and temperature control. All animals were given access to tap water and laboratory chow ad libitum.
Extracted molecule total RNA
Extraction protocol kit: QIAGEN, Rneasy Mini
Label Cy3
Label protocol Manual: Low RNA Input Linear Amplification kit standard protocol
 
Channel 2
Source name Control Cy5
Organism Mus musculus
Characteristics strain: C57BL/6J
tissue: brain
treatment: sham
time: 6h
Treatment protocol Saline control 6 h
Growth protocol Forty male mice (8 to 12-weeks-old, 25 ~ 35 g body weight) were housed at the Animal Institution in Showa University in acrylic cages (8 mice / cage) at 23ºC, and maintained with a standard 12 h light/dark cycle with optimum humidity and temperature control. All animals were given access to tap water and laboratory chow ad libitum.
Extracted molecule total RNA
Extraction protocol kit: QIAGEN, Rneasy Mini
Label Cy5
Label protocol Manual: Low RNA Input Linear Amplification kit standard protocol
 
 
Hybridization protocol hybridization_Kit: in situ hybridization plus
Scan protocol Scanner: Agilent DNA microarray Scaner
Description Dye-swap (Cy5/Cy3) of 6 h Brain Sample 2
Data processing Software: Feature Extraction ver 9.5.3.1
 
Submission date Mar 28, 2011
Last update date Apr 01, 2012
Contact name RANDEEP RAKWAL
E-mail(s) plantproteomics@gmail.com
Phone +81-(0)90-1853-7875
Organization name University of Tsukuba
Department Institute of Health and Sport Sciences
Lab Global Sport Innovation (GSI) 403
Street address 1-1-1 Tennodai
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8574
Country Japan
 
Platform ID GPL4134
Series (1)
GSE28201 DNA Microarray Analysis Approach for Unraveling the Ischemic Mouse Brain Transcriptome

Data table header descriptions
ID_REF
VALUE log 10 normalized treatment/control
Norm_Ch1 Normalized (lowess and rank consistency) signal of ch1
Norm_Ch2 Normalized (lowess and rank consistency) signal of ch2
Raw_Ch1 The net CH1 signal following the subtraction of the background from the raw mean CH1 signal
Raw_Ch2 The net CH2 signal following the subtraction of the background from the raw mean CH2 signal

Data table
ID_REF VALUE Norm_Ch1 Norm_Ch2 Raw_Ch1 Raw_Ch2
1 1.05 24781.46 26060.68 12686.6 49668.3
2 0.85 3.53 2.99 -0.58 0.46
3 0.85 3.51 2.99 2.21 -0.67
4 0.85 3.5 2.98 -0.68 -3.02
5 0.86 3.48 2.98 -3.59 -0.27
6 0.86 3.47 2.98 0.46 -5.37
7 0.86 3.46 2.97 1.38 -4.06
8 0.86 3.45 2.97 1.23 -1.72
9 0.86 3.43 2.96 -1.69 0.49
10 0.86 3.42 2.96 -0.79 -0.78
11 0.87 3.41 2.95 -0.21 -5.8
12 0.87 3.4 2.95 1.41 -4.6
13 0.74 137.29 101.76 81.52 177.33
14 0.87 3.39 2.94 1.18 0.97
15 0.98 47.72 46.98 29.12 80.27
16 0.68 284.94 192.82 167.82 342.34
18 0.87 3.36 2.93 -1.29 1.91
19 1.41 33.11 46.69 20.42 79.78
20 0.77 11.2 8.62 7.34 13.89
21 0.89 42.58 37.78 26.29 64.7

Total number of rows: 45018

Table truncated, full table size 1593 Kbytes.




Supplementary file Size Download File type/resource
GSM698133_US22502699_251486831690_S01_GE2_v5_95_Feb07_1_2.txt.gz 15.5 Mb (ftp)(http) TXT
Processed data included within Sample table
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