|
| Status |
Public on Apr 01, 2012 |
| Title |
SHAM(Cy3) x PMCAO (Cy5) 24h |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Control Cy3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: brain
treatment: sham
time: 24h
|
| Treatment protocol |
Saline control 24 h
|
| Growth protocol |
Forty male mice (8 to 12-weeks-old, 25 ~ 35 g body weight) were housed at the Animal Institution in Showa University in acrylic cages (8 mice / cage) at 23ºC, and maintained with a standard 12 h light/dark cycle with optimum humidity and temperature control. All animals were given access to tap water and laboratory chow ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
kit: QIAGEN, Rneasy Mini
|
| Label |
Cy3
|
| Label protocol |
Manual: Low RNA Input Linear Amplification kit standard protocol
|
| |
|
| Channel 2 |
| Source name |
Treatment Cy5
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: brain
treatment: permanent middle cerebral artery occlusion
time: 24h
|
| Treatment protocol |
PMCAO control 24 h
|
| Growth protocol |
Forty male mice (8 to 12-weeks-old, 25 ~ 35 g body weight) were housed at the Animal Institution in Showa University in acrylic cages (8 mice / cage) at 23ºC, and maintained with a standard 12 h light/dark cycle with optimum humidity and temperature control. All animals were given access to tap water and laboratory chow ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
kit: QIAGEN, Rneasy Mini
|
| Label |
Cy5
|
| Label protocol |
Manual: Low RNA Input Linear Amplification kit standard protocol
|
| |
|
| |
| Hybridization protocol |
hybridization_Kit: in situ hybridization plus
|
| Scan protocol |
Scanner: Agilent DNA microarray Scaner
|
| Description |
Dye-swap (Cy3/Cy5) of 24 h Brain Sample 1
|
| Data processing |
Software: Feature Extraction ver 9.5.3.1
|
| |
|
| Submission date |
Mar 28, 2011 |
| Last update date |
Apr 01, 2012 |
| Contact name |
RANDEEP RAKWAL |
| E-mail(s) |
plantproteomics@gmail.com
|
| Phone |
+81-(0)90-1853-7875
|
| Organization name |
University of Tsukuba
|
| Department |
Institute of Health and Sport Sciences
|
| Lab |
Global Sport Innovation (GSI) 403
|
| Street address |
1-1-1 Tennodai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8574 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE28201 |
DNA Microarray Analysis Approach for Unraveling the Ischemic Mouse Brain Transcriptome |
|
