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Status |
Public on Aug 04, 2011 |
Title |
peritumoral HCC, non-lymph node metastasis, patient 34 |
Sample type |
RNA |
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Source name |
peritumoral HCC, NLNM, patient 34
|
Organism |
Homo sapiens |
Characteristics |
individual: patient 34 disease state: hepatocellular carcinoma (HCC) tissue: peritumoral HCC tissues metastasis type: non-lymph node metastasis (NLNM)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was purified from the tissue specimens using the High Pure RNA Paraffin Kit (Roche Diagnostics, Germany) according to the manufacturer’s protocol.Proteinase K digestion time was 12 hours for each sample. RNA samples were stored at -80°C until use.
|
Label |
Cy3, Cy5
|
Label protocol |
RNA with a cycle threshold (Ct) value ≤28 was used for the DASL assay.
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|
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Hybridization protocol |
Standard Illumina hybridization protocol
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Scan protocol |
Standard Illumina scanning protocol
|
Description |
formalin-fixed, paraffin-embedded specimen peritumoral tissues of patient 34 peritumoral tissues NLNM
|
Data processing |
Scanned data were uploaded into GenomeStudio for further analysis.
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Submission date |
Mar 29, 2011 |
Last update date |
Aug 04, 2011 |
Contact name |
Zuolin Xiang |
E-mail(s) |
xzl815@sohu.com
|
Organization name |
Zhongshan Hospital, Fudan University
|
Street address |
136 Yi Xue Yuan Road
|
City |
Shanghai |
ZIP/Postal code |
200032 |
Country |
China |
|
|
Platform ID |
GPL5858 |
Series (1) |
GSE28248 |
Gene Expression Profiling of Fixed Tissues of Lymph Node Metastasis in Hepatocellular Carcinoma |
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