|
| Status |
Public on Jun 01, 2011 |
| Title |
iPSC cultured with feeder free condition rep2 |
| Sample type |
RNA |
| |
|
| Source name |
iPSC cultured with feeder free condition rep2
|
| Organism |
Mus musculus |
| Characteristics |
culture condition: iPSC cultured with feeder free condition
strain: C57BL/6
cell type: induced-pluripotent stem cells
|
| Biomaterial provider |
BRC and ECACC
|
| Treatment protocol |
Cells were dissociating with 0.25% trypsin and lysised in Trizol solution.
|
| Growth protocol |
Cells in the feeder condition were cultured on mitomycin C -treated SNL76/7 cells . miPS cells under feeder-free condition were cultured without feeder cells after adding 1000 units/ml of LIF into the media containing 15% FBS, 0.1mM NEAA, 0.1mM 2-Mercaptoethanol in DMEM (high glucose without sodium pyruvate)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified for expression analysis by RNeasy mini kit (Qiagen, Washington DC, MA, http://www.qiagen.com/) after DNaseI treatment.
|
| Label |
cy3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
miPS.FF2
|
| Data processing |
Quantile normalization of Illumina data and B-statistic calculations were carried out using the lumi and limma packages of Bioconductor in the R statistical language.
|
| |
|
| Submission date |
Mar 30, 2011 |
| Last update date |
Jun 01, 2011 |
| Contact name |
Yuki Hasegawa |
| E-mail(s) |
hasegawa@gsc.riken.jp
|
| Phone |
+81-45-503-9222
|
| Fax |
+81-45-503-9216
|
| Organization name |
RIKEN
|
| Department |
OSC
|
| Lab |
LSA technology development unit
|
| Street address |
1-7-22
|
| City |
Suehiro-cho, Tsurumi-ku |
| State/province |
Yokohama-city |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE28262 |
CC chemokine ligand 2 and LIF cooperatively promote pluripotency in mouse induced pluripotent cells |
|
