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Sample GSM7000671 Query DataSets for GSM7000671
Status Public on Feb 05, 2024
Title Perkinsus_marinus_1
Sample type SRA
Source name Perkinsus marinus
Organism Perkinsus marinus
Characteristics assay: PRO-seq
degron type: none
dtag-13 treatment: untreated
heat shock: no
replicate: 1
Extracted molecule total RNA
Extraction protocol RNA extraction, run-on, and library preparation were executed according to “Mahat, D. B. et al. Base-pair-resolution genome-wide mapping of active RNA polymerases using precision nuclear run-on (PRO-seq). Nat. Protoc. 11, 1455–1476 (2016).” (PMID: 27442863).
Library prep was performed using Illumina custom adapters: Reverse 3' RNA adapter (/5Phos/NNNNNNGAUCGUCGGACUGUAGAACUCUGAAC/3InvdT) and Reverse 5' RNA Adapter (5'-CCUUGGCACCCGAGAAUUCCA-3').
Library strategy OTHER
Library source transcriptomic
Library selection other
Instrument model Illumina NextSeq 500
Data processing FASTQ data was aligned and processed using the proseq2.0 pipeline developed by Danko lab (
The mESC data was competitively aligned to a merged genome assembly of mm10 and dm3. Merging the two genome builds was performed using fastq merge.
Submission date Jan 28, 2023
Last update date Feb 05, 2024
Contact name Alexandra Georgiana Chivu
Phone 6072624972
Organization name Cornell University
Department Molecular Biology and Genetics
Lab Danko and Lis labs
Street address 215 Tower Rd
City Ithaca
State/province NY
ZIP/Postal code 14853
Country USA
Platform ID GPL33068
Series (1)
GSE223913 Pol II pausing is a milestone on the road to complex animals
BioSample SAMN32949458
SRA SRX19205925

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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