|
Status |
Public on Jan 29, 2024 |
Title |
5637_PAI1rs1050813_1 |
Sample type |
RNA |
|
|
Source name |
Human bladder cancer cell
|
Organism |
Homo sapiens |
Characteristics |
cell line: 5637 cell type: Human bladder cancer cell transfection plasmid: PAI1 rs1050813 mutant
|
Treatment protocol |
PAI1-WT-pDEST26, PAI1- rs7242-pDEST26, or PAI1-rs1050813-pDEST26 were used as plasmid DNA. C125E01 in pDONR223 (Kind gift from Dr. Dominic Esposito, NIH/NCI) was also subcloned into pDEST26 (C125E01-pDEST26) and this plasmid was used as a negative control. Two µg of plasmid DNA was used for plasmid transfection using jetPRIME.
|
Growth protocol |
UROtsa and 5637 were cultured in low glucose DMEM or RPMI1640 containing 10% FBS, 1% Penicillin-Streptomycin and incubated at 37°C and 5% CO2.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from cells using RNeasy Plus Mini Kit (Qiagen).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared from 0.55 ug total RNA using the Illumina TotalPrep RNA Amplification Kit (Ambion, Austin,TX)
|
|
|
Hybridization protocol |
Following fragmentation, 0.75 ug of cRNA were hybridized to the Illumina Expression Beadchip according to the protocols provided by the manufacturer
|
Scan protocol |
Arrays were scanned using the Illumina Bead Array Reader Confocal Scanner
|
Description |
N_8B_1
|
Data processing |
Array data export processing and analysis was performed using Illumina GenomeStudio v2011.1 (Gene Expression Module v1.9.0)
|
|
|
Submission date |
Feb 01, 2023 |
Last update date |
Jan 29, 2024 |
Contact name |
Hideki Furuya |
E-mail(s) |
hideki.furuya@cshs.org
|
Phone |
3104234607
|
Organization name |
Cedars Sinai Medical Center
|
Department |
Surgery
|
Street address |
110 N. George Burns Road, Davis 2025
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90048 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE224309 |
Association of SNPs in the PAI1 Gene with Disease Recurrence and Clinical Outcome in Bladder Cancer |
|