|
| Status |
Public on Jan 29, 2024 |
| Title |
5637_PAI1rs1050813_2 |
| Sample type |
RNA |
| |
|
| Source name |
Human bladder cancer cell
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: 5637
cell type: Human bladder cancer cell
transfection plasmid: PAI1 rs1050813 mutant
|
| Treatment protocol |
PAI1-WT-pDEST26, PAI1- rs7242-pDEST26, or PAI1-rs1050813-pDEST26 were used as plasmid DNA. C125E01 in pDONR223 (Kind gift from Dr. Dominic Esposito, NIH/NCI) was also subcloned into pDEST26 (C125E01-pDEST26) and this plasmid was used as a negative control. Two µg of plasmid DNA was used for plasmid transfection using jetPRIME.
|
| Growth protocol |
UROtsa and 5637 were cultured in low glucose DMEM or RPMI1640 containing 10% FBS, 1% Penicillin-Streptomycin and incubated at 37°C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using RNeasy Plus Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 0.55 ug total RNA using the Illumina TotalPrep RNA Amplification Kit (Ambion, Austin,TX)
|
| |
|
| Hybridization protocol |
Following fragmentation, 0.75 ug of cRNA were hybridized to the Illumina Expression Beadchip according to the protocols provided by the manufacturer
|
| Scan protocol |
Arrays were scanned using the Illumina Bead Array Reader Confocal Scanner
|
| Description |
N_8B_2
|
| Data processing |
Array data export processing and analysis was performed using Illumina GenomeStudio v2011.1 (Gene Expression Module v1.9.0)
|
| |
|
| Submission date |
Feb 01, 2023 |
| Last update date |
Jan 29, 2024 |
| Contact name |
Hideki Furuya |
| E-mail(s) |
hideki.furuya@cshs.org
|
| Phone |
3104234607
|
| Organization name |
Cedars Sinai Medical Center
|
| Department |
Surgery
|
| Street address |
110 N. George Burns Road, Davis 2025
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90048 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE224309 |
Association of SNPs in the PAI1 Gene with Disease Recurrence and Clinical Outcome in Bladder Cancer |
|
