|
| Status |
Public on Aug 07, 2023 |
| Title |
287_6 |
| Sample type |
SRA |
| |
|
| Source name |
Human cells in mouse xenograft
|
| Organisms |
Homo sapiens; Mus musculus |
| Characteristics |
tissue: Meningies
cell line: CH-157MN
cell type: Human Meningioma
genotype: Merlin rescue
treatment: 200ug/ml doxycycline
|
| Extracted molecule |
total RNA |
| Extraction protocol |
cell dissociation using collagenase and Trypsin
10X genomics single cell 3' kit, v3.1
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Merlin rescue in meningioma mouse xenografts
10X Genomics
|
| Data processing |
Cell ranger v6.1.2
R studio, v4.2.1
Seurat v4.3.0
SCTransform, v0.3.5
Assembly: hg19 & mm10
Supplementary files format and content: Tab-separated values files and matrix files
|
| |
|
| Submission date |
Feb 02, 2023 |
| Last update date |
Aug 07, 2023 |
| Contact name |
Charlotte Eaton |
| E-mail(s) |
charlotte.eaton@UCSF.edu
|
| Organization name |
UCSF
|
| Street address |
1350 3rd St.
|
| City |
San Francisco |
| ZIP/Postal code |
94117 |
| Country |
USA |
| |
|
| Platform ID |
GPL25526 |
| Series (1) |
| GSE224347 |
Merlin rescue drives Wnt signalling |
|
| Relations |
| BioSample |
SAMN33020654 |
| SRA |
SRX19260880 |
