|
| Status |
Public on Feb 26, 2024 |
| Title |
genomic DNA from Tet1-KO sperm 2 |
| Sample type |
genomic |
| |
|
| Source name |
KO sperm
|
| Organism |
Mus musculus |
| Characteristics |
genotype: KO
tissue: sperm
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted using standard Phenol:Chloform extraction
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented, and hybridized to Illumina Infinium Mouse285 Methylation BeadChip 12v1-0 using standard Illumina Protocol
|
| Scan protocol |
Illumina iScan System
|
| Description |
Bisulfite treated
KO-5333
|
| Data processing |
SeSAMe R Package
Beta values of individual probe for each individual sample. Probes that did not pass SeSAMe quality control pOOBAH approach is maksed with NA
|
| |
|
| Submission date |
Feb 02, 2023 |
| Last update date |
Feb 26, 2024 |
| Contact name |
Rexxi Diptya Prasasya |
| E-mail(s) |
rexxi.prasasya@pennmedicine.upenn.edu
|
| Organization name |
University of Pennsylvania
|
| Department |
Cell and Developmental Biology
|
| Lab |
Bartolomei Lab
|
| Street address |
3400 Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL31950 |
| Series (2) |
| GSE224349 |
TET1 catalytic activity is required for reprogrammign of imprinting control regions and the patterning of sperm-specific hypomethylated regions [Mouse Methylation285 12v1-0 BeadChip] |
| GSE224459 |
TET1 catalytic activity is required for reprogrammign of imprinting control regions and the patterning of sperm-specific hypomethylated regions |
|
