|
Status |
Public on Feb 26, 2024 |
Title |
genomic DNA from Tet1-KO sperm 4 |
Sample type |
genomic |
|
|
Source name |
KO sperm
|
Organism |
Mus musculus |
Characteristics |
genotype: KO tissue: sperm
|
Extracted molecule |
genomic DNA |
Extraction protocol |
genomic DNA was extracted using standard Phenol:Chloform extraction
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented, and hybridized to Illumina Infinium Mouse285 Methylation BeadChip 12v1-0 using standard Illumina Protocol
|
Scan protocol |
Illumina iScan System
|
Description |
Bisulfite treated KO_REP
|
Data processing |
SeSAMe R Package Beta values of individual probe for each individual sample. Probes that did not pass SeSAMe quality control pOOBAH approach is maksed with NA
|
|
|
Submission date |
Feb 02, 2023 |
Last update date |
Feb 26, 2024 |
Contact name |
Rexxi Diptya Prasasya |
E-mail(s) |
rexxi.prasasya@pennmedicine.upenn.edu
|
Organization name |
University of Pennsylvania
|
Department |
Cell and Developmental Biology
|
Lab |
Bartolomei Lab
|
Street address |
3400 Civic Center Blvd
|
City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19104 |
Country |
USA |
|
|
Platform ID |
GPL31950 |
Series (2) |
GSE224349 |
TET1 catalytic activity is required for reprogrammign of imprinting control regions and the patterning of sperm-specific hypomethylated regions [Mouse Methylation285 12v1-0 BeadChip] |
GSE224459 |
TET1 catalytic activity is required for reprogrammign of imprinting control regions and the patterning of sperm-specific hypomethylated regions |
|