|
| Status |
Public on Jun 30, 2024 |
| Title |
85sdd rep3 |
| Sample type |
SRA |
| |
|
| Source name |
seedling
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype: ski2 dcl4 dcl2
age: 7-d-old
stress: 85 min with heat stress
|
| Treatment protocol |
HS was imposed by submersion of parafilm-sealed plates into a temperature_x0002_controlled water bath
|
| Growth protocol |
Surface sterilized seeds were imbibed in water at 4 °C for 3 days and germinated on 1/2 MS agar at standard condition (16h ligth/8h dark, 22 °C) for 7 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol (Invitrogen)
stander Illumina mRNA-seq protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
85sdd#2
|
| Data processing |
Bowtie for alignment, v2, default parameter
Cufflink for expression profiling, V2.1.0, default parameter
Assembly: TAIR11
Supplementary files format and content: pyj_raw_data.xlsx represents FPKMs
|
| |
|
| Submission date |
Mar 30, 2023 |
| Last update date |
Jun 30, 2024 |
| Contact name |
Yan wei |
| E-mail(s) |
yanwei_bio@126.com
|
| Organization name |
Southern University of Science and Technology
|
| Street address |
shenzhenxueyuanroad
|
| City |
Shenzhen |
| ZIP/Postal code |
518000 |
| Country |
China |
| |
|
| Platform ID |
GPL23157 |
| Series (1) |
| GSE228589 |
SMXL4/5-HsfA2 regulatory loop orchestrates plant growth-thermotolerance trade-off |
|
| Relations |
| BioSample |
SAMN33995924 |
| SRA |
SRX19832242 |
