|
| Status |
Public on Dec 31, 2011 |
| Title |
ABADFPM_3 |
| Sample type |
RNA |
| |
|
| Source name |
whole seedling treated with abscisic acid and DFPM
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col0
age: 12 day old plate grown seedling
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol (Invitrogen, Carlsbad, CA, USA) and further purified using RNeasy Plant RNA purification kit (QIAgen, Valencia, CA, USA)
|
| Label |
Biotin
|
| Label protocol |
UCSD GeneChip Microarray Core; Affymetrix GeneChip IVT Labeling Kit, according to manufactuer's protocol (GeneChip Expression Analysis Technical Manual)
|
| |
|
| Hybridization protocol |
UCSD GeneChip Microarray Core; Affymetrix Eukaryotic Target Hybridization protocol (GeneChip Expression Analysis Technical Manual)
|
| Scan protocol |
UCSD GeneChip Microarray Core; Affymetrix GeneChip Scanner 3000 with GCOS software according to manufactuer's protocol (GeneChip Expression Analysis Technical Manual)
|
| Description |
Plate grown 12-day-old seedlings were transferred into 6 well plates with 30 μM DFPM and 10 μM ABA in water as a treatment for 6 hours. DFPM was added 30 min prior to ABA treatment.
|
| Data processing |
Probe set summarization and normalization was performed by robust multi-array averaging (gcrma, including background subtraction, summarization by median polish and quantile normalization) using the R/Bioconductor package affy.
|
| |
|
| Submission date |
Apr 21, 2011 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Julian Schroeder |
| URL |
http://www-biology.ucsd.edu/labs/schroeder/index.html
|
| Organization name |
UCSD
|
| Department |
Division of Biological Sciences
|
| Lab |
Schroeder Laboratory
|
| Street address |
9500 Gilman Drive #0116
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0116 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE28800 |
Gene expression data from ABA, [5-(3,4-Dichlorophenyl)Furan-2-yl]-Piperidin-1-ylMethanethione (DFPM), and ABA+DFPM treated Arabidopsis thaliana seedlings. |
|
| Relations |
| Reanalyzed by |
GSE118579 |
