|
| Status |
Public on Oct 01, 2023 |
| Title |
MPF-R2 RET |
| Sample type |
RNA |
| |
|
| Source name |
MPFR cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line background: MCF-7 cells
cell type: MPFR cells
genotype: RET siRNA
|
| Treatment protocol |
MPFR and TPFR were established through long-term treatment with 100 nM fulvestrant and 200nM palbociclib over a period of 2-4 months.
|
| Growth protocol |
MPFR cells were cultured in phenol red-free Dulbecco’s modified Eagle medium (D-MEM)/F-12 (Gibco) supplemented with 1% heat-inactivated fetal bovine serum (FBS, Gibco), 2 mM glutamine (Gibco) and 6 ng/ml insulin (Sigma-Aldrich) supplemented with 200 nM CDK4/6i and 100 nM fulvestrant. TPFR cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 media without phenol red (Gibco, ThermoFisher Scientific, Waltham, MA, USA), supplemented with 5% FBS, 2 mmol/L glutamine, 8 µg /mlL insulin and 200 nM CDK4/6i and 100 nM fulvestrant.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified with RNeasy Mini kit (Qiagen) according to the manufacturer´s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on human transcriptome array 2.0 (HTA). GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from MPFR RET-siRNA
CAlv-Ext-MPF-R2-ret-HTA-2_0-a1.sst-rma-combined-dabg.chp Signal
|
| Data processing |
Data analysis was conducted in Transcriptome Analysis Console (Thermo Fisher Scientific).
|
| |
|
| Submission date |
Mar 31, 2023 |
| Last update date |
Oct 01, 2023 |
| Contact name |
Carlos Maria Lourenco Alves |
| E-mail(s) |
calves@health.sdu.dk
|
| Phone |
+4591101707
|
| Organization name |
SDU
|
| Street address |
J.B. Winsløws vej 25 3.
|
| City |
Odense |
| ZIP/Postal code |
5000 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE228637 |
Microarray gene expression data from RET siRNA-mediated knockdown in CDK4/6 inhibitor breast cancer resistant cells |
|
