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Series GSE228637 Query DataSets for GSE228637
Status Public on Oct 01, 2023
Title Microarray gene expression data from RET siRNA-mediated knockdown in CDK4/6 inhibitor breast cancer resistant cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary RET is overexpressed in breast cancer cell lines resistant to combined CDK4/6i and endocrine therapy. siRNA-mediated knockdown of RET was performed to identify main pathways altered upon RET-knockdown.
We used microarrays to detail the global programme of gene expression following RET knockdown in 2 cell models resistant to combined CDK4/6 inhibitor palbociclib and fulvestrant (MPFR and TPFR). MPFR-ret and TPFR-ret were compared to MPFR-ctrl and TPFR-ctrl, respectively. The gene expression analysis revealed a significant number of genes altered in the knockdown (ret) vs. ctrl
Overall design Combined palbociclib and fulvestrant resistant cell lines were derived from MCF-7 and T47D cells by long-term treatment with hig concentration of palbociclib and fulvestrant. RET knockdown was performed by chemical transfection with lipofectime with siRNAs targeting RET and scramble siRNA as control. Total RNA from 3 biological replicates of RET-siRNA and control in MPFR and TPFR cells was extracted and arrayed separately in Human Transcriptome Array 2.0 (HTA, Affymetrix).
Contributor(s) Carla AL
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Submission date Mar 31, 2023
Last update date Oct 02, 2023
Contact name Carlos Maria Lourenco Alves
Phone +4591101707
Organization name SDU
Street address J.B. Winsløws vej 25 3.
City Odense
ZIP/Postal code 5000
Country Denmark
Platforms (1)
GPL17586 [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version]
Samples (12)
GSM7134252 MPF-R1 control
GSM7134253 MPF-R1 RET
GSM7134254 MPF-R2 control
BioProject PRJNA950908

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE228637_RAW.tar 321.1 Mb (http)(custom) TAR (of CEL)
GSE228637_all_transcripts_MPFR_KDvsCTRL.xlsx 2.1 Mb (ftp)(http) XLSX
GSE228637_all_transcripts_TPFR_KDvsCTRL.xlsx 2.2 Mb (ftp)(http) XLSX
Processed data included within Sample table
Processed data are available on Series record

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