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| Status |
Public on Oct 01, 2023 |
| Title |
Microarray gene expression data from RET siRNA-mediated knockdown in CDK4/6 inhibitor breast cancer resistant cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
RET is overexpressed in breast cancer cell lines resistant to combined CDK4/6i and endocrine therapy. siRNA-mediated knockdown of RET was performed to identify main pathways altered upon RET-knockdown.
We used microarrays to detail the global programme of gene expression following RET knockdown in 2 cell models resistant to combined CDK4/6 inhibitor palbociclib and fulvestrant (MPFR and TPFR). MPFR-ret and TPFR-ret were compared to MPFR-ctrl and TPFR-ctrl, respectively. The gene expression analysis revealed a significant number of genes altered in the knockdown (ret) vs. ctrl
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| Overall design |
Combined palbociclib and fulvestrant resistant cell lines were derived from MCF-7 and T47D cells by long-term treatment with hig concentration of palbociclib and fulvestrant. RET knockdown was performed by chemical transfection with lipofectime with siRNAs targeting RET and scramble siRNA as control. Total RNA from 3 biological replicates of RET-siRNA and control in MPFR and TPFR cells was extracted and arrayed separately in Human Transcriptome Array 2.0 (HTA, Affymetrix).
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| Contributor(s) |
Carla AL |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 31, 2023 |
| Last update date |
Oct 02, 2023 |
| Contact name |
Carlos Maria Lourenco Alves |
| E-mail(s) |
calves@health.sdu.dk
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| Phone |
+4591101707
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| Organization name |
SDU
|
| Street address |
J.B. Winsløws vej 25 3.
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| City |
Odense |
| ZIP/Postal code |
5000 |
| Country |
Denmark |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA950908 |
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