|
Status |
Public on Apr 05, 2023 |
Title |
mPFC, sal_f1 |
Sample type |
SRA |
|
|
Source name |
medial prefrontal cortex
|
Organism |
Rattus norvegicus |
Characteristics |
Sex: Female strain: Sprague Dawley tissue: medial prefrontal cortex age: P45 treatment: Saline
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from bilateral mPFC using RNAeasy kit (Qiagen). Only RNA with RIN > 8.0 were used for RNA-seq. oligo-dT
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Basecalling with Illumina RTA 1.9 Demultiplexing with Illumina bcl2fastq2 Fastq files were combined, aligned, and quantitated with Illumina's DRAGEN Bio-IT system version 3.9.3-8 Assembly: Ensembl Release 101 Rnor_6.0.101 Supplementary files format and content: Gene counts with TPM in tab-delimitted text format
|
|
|
Submission date |
Apr 04, 2023 |
Last update date |
Apr 05, 2023 |
Contact name |
Arvind Palanisamy |
E-mail(s) |
arvind.palanisamy@wustl.edu
|
Organization name |
Washington University School of Medicine
|
Department |
Anesthesiology
|
Street address |
660 S Euclid Ave
|
City |
St. Louis |
State/province |
MO |
ZIP/Postal code |
63110 |
Country |
USA |
|
|
Platform ID |
GPL25947 |
Series (1) |
GSE228913 |
Transcriptomic changes in the medial prefrontal cortex of juvenile rat offspring after induced birth with oxytocin |
|
Relations |
BioSample |
SAMN34066525 |
SRA |
SRX19862539 |