|
| Status |
Public on Apr 05, 2023 |
| Title |
mPFC, oxt_m3 |
| Sample type |
SRA |
| |
|
| Source name |
medial prefrontal cortex
|
| Organism |
Rattus norvegicus |
| Characteristics |
Sex: Male
strain: Sprague Dawley
tissue: medial prefrontal cortex
age: P45
treatment: Oxytocin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from bilateral mPFC using RNAeasy kit (Qiagen). Only RNA with RIN > 8.0 were used for RNA-seq.
oligo-dT
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Basecalling with Illumina RTA 1.9
Demultiplexing with Illumina bcl2fastq2
Fastq files were combined, aligned, and quantitated with Illumina's DRAGEN Bio-IT system version 3.9.3-8
Assembly: Ensembl Release 101 Rnor_6.0.101
Supplementary files format and content: Gene counts with TPM in tab-delimitted text format
|
| |
|
| Submission date |
Apr 04, 2023 |
| Last update date |
Apr 05, 2023 |
| Contact name |
Arvind Palanisamy |
| E-mail(s) |
arvind.palanisamy@wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Department |
Anesthesiology
|
| Street address |
660 S Euclid Ave
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL25947 |
| Series (1) |
| GSE228913 |
Transcriptomic changes in the medial prefrontal cortex of juvenile rat offspring after induced birth with oxytocin |
|
| Relations |
| BioSample |
SAMN34066521 |
| SRA |
SRX19862543 |
