|
| Status |
Public on Jul 01, 2011 |
| Title |
sch9-as dot6 tod6 + PP1 |
| Sample type |
SRA |
| |
|
| Source name |
sch9-as dot6 tod6 whole cells, PP1
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: AH347
genotype: TB50alpha sch9-as::pRS304 dot6::KanMX6 tod6::BleMX6
tissue: whole cell
treatment: 300 nM 1NM-PP1 30 min
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracts were purified of genomic DNA contaminations using RNeasy kits (QIAGen). Libraries were prepared from pools of equivalent amounts of RNA from four independent experiments and sequenced using version 4 kits and single read flow cells on a Genome Analyzer IIx machine according to the manufacturerâs instructions (Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer IIx |
| |
|
| Description |
mRNA (polyA+)
|
| Data processing |
Reads were mapped to unique portions of the transcriptome of S. cerevisiae. Their numbers for each mRNA was normalized to the total number of aligned reads and to the length of the unique sequence of the mRNA.
|
| |
|
| Submission date |
May 06, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Jacques Rougemont |
| E-mail(s) |
jacques.rougemont@epfl.ch
|
| Organization name |
EPFL
|
| Department |
School of Life Sciences
|
| Lab |
Bioinformatics and Biostatistics Core Facility
|
| Street address |
Station 15
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL13272 |
| Series (2) |
| GSE29122 |
Sch9 regulates ribosome biogenesis via Stb3, Dot6 and Tod6 and the histone deacetylase complex RPD3L (mRNA-Seq data) |
| GSE29125 |
Sch9 regulates ribosome biogenesis via Stb3, Dot6 and Tod6 and the histone deacetylase complex RPD3L |
|
| Relations |
| SRA |
SRX062012 |
| BioSample |
SAMN00264848 |
