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Sample GSM721168 Query DataSets for GSM721168
Status Public on Jul 01, 2011
Title sch9-as dot6 tod6 + PP1
Sample type SRA
 
Source name sch9-as dot6 tod6 whole cells, PP1
Organism Saccharomyces cerevisiae
Characteristics strain: AH347
genotype: TB50alpha sch9-as::pRS304 dot6::KanMX6 tod6::BleMX6
tissue: whole cell
treatment: 300 nM 1NM-PP1 30 min
Extracted molecule total RNA
Extraction protocol RNA extracts were purified of genomic DNA contaminations using RNeasy kits (QIAGen). Libraries were prepared from pools of equivalent amounts of RNA from four independent experiments and sequenced using version 4 kits and single read flow cells on a Genome Analyzer IIx machine according to the manufacturer’s instructions (Illumina).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina Genome Analyzer IIx
 
Description mRNA (polyA+)
Data processing Reads were mapped to unique portions of the transcriptome of S. cerevisiae. Their numbers for each mRNA was normalized to the total number of aligned reads and to the length of the unique sequence of the mRNA.
 
Submission date May 06, 2011
Last update date May 15, 2019
Contact name Jacques Rougemont
E-mail(s) jacques.rougemont@epfl.ch
Organization name EPFL
Department School of Life Sciences
Lab Bioinformatics and Biostatistics Core Facility
Street address Station 15
City Lausanne
ZIP/Postal code 1015
Country Switzerland
 
Platform ID GPL13272
Series (2)
GSE29122 Sch9 regulates ribosome biogenesis via Stb3, Dot6 and Tod6 and the histone deacetylase complex RPD3L (mRNA-Seq data)
GSE29125 Sch9 regulates ribosome biogenesis via Stb3, Dot6 and Tod6 and the histone deacetylase complex RPD3L
Relations
SRA SRX062012
BioSample SAMN00264848

Supplementary file Size Download File type/resource
GSM721168_4b.bam 326.4 Mb (ftp)(http) BAM
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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