|
| Status |
Public on Oct 26, 2023 |
| Title |
lens, 8weeks-3 |
| Sample type |
RNA |
| |
|
| Source name |
ICR rat lens, 8weeks of age
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: ICR/Ihr
tissue: lens
time: 8weeks
condition: cortical opacity
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The lens removed from the culture medium was ground with a biosmasher until it fell apart. Then treat the sample with TRIZOL, cDNA synthesis and amplification was performed
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99° for 5 minutes, then 45° for 5 minutes centrifuge at max speed for 1 minute. • Transfer 130μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 16 hours at 45° at 60rpm• Fluidics washing is FS450_0002
|
| Scan protocol |
Gene ChIP Scanner 3000 7G System
|
| Description |
ICR rat lens, 8weeks of age
|
| Data processing |
Data were processed using R. MAT background correction were applied to probe intensities, gene level expression were summarized from the corrected intensities and then quantile normalized.
RaGene-2_0-st.pgf
RaGene-2_0-st.mps
|
| |
|
| Submission date |
Apr 21, 2023 |
| Last update date |
Oct 26, 2023 |
| Contact name |
Masaru Takashima |
| Organization name |
University of Fukui
|
| Street address |
3-9-1 Bunkyo
|
| City |
Fukui |
| ZIP/Postal code |
910-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17117 |
| Series (2) |
| GSE230322 |
Microarray data from 4-, 8-, and 10-week-old ICR lenses |
| GSE230323 |
Microarray data of ICR lenses |
|
