|
| Status |
Public on Oct 01, 2011 |
| Title |
Cyclopamine 5 |
| Sample type |
RNA |
| |
|
| Source name |
zebrafish embryo
|
| Organism |
Danio rerio |
| Characteristics |
exposure time: 24 h
treatment: Cyclopamine_10µM
|
| Treatment protocol |
100 embryos per treatment, treatments: control, 10 µM Cyclopamine (C), 10 µM SANT-2 (S), 10 µM GANT-61 (G), 24 h (0.5 - 24 hpf), respectively; all in 5 independent replicates
|
| Growth protocol |
Zebrafish embryos were collected directly after fertilisation and treated starting 0.5 hpf in exposure medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Synthesis of cDNA, cRNA and cRNA-labeling was performed with the Agilent One-Color Quick Amp Labeling Kit according to the manufacturer’s instructions. cRNA was labelled with Cy3.
|
| |
|
| Hybridization protocol |
Hybridization was performed using the Agilent Gene Expression Hybridization Kit following manufacturer's instructions.
|
| Scan protocol |
Scanned on an Agilent scanner. Images were quantified using Agilent Feature Extraction Software (version 10.1.1.1).
|
| Description |
Gene expression of control embryos treated with Cyclopamine for 24 h (0.5hpf - 24hpf)
|
| Data processing |
Feature extraction files (processed signals) were used for statistics, quantile normalization of all processed signals before significance testing.
|
| |
|
| Submission date |
May 17, 2011 |
| Last update date |
Oct 01, 2011 |
| Contact name |
Wibke Busch |
| E-mail(s) |
wibke.busch@ufz.de
|
| Phone |
00493412351515
|
| Organization name |
UFZ Helmholtz Centre for Environmental Research
|
| Department |
Bioanalytical Ecotoxicology
|
| Lab |
Scholz
|
| Street address |
Permoser Str. 15
|
| City |
Leipzig |
| ZIP/Postal code |
04318 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13526 |
| Series (1) |
| GSE29357 |
Zebrafish embryos exposed to the model hedgehog pathway inhibitor cyclopamine, as well as to the potential hedghog pathway disrupters SANT-2 and GANT-61. |
|
