|
| Status |
Public on May 01, 2024 |
| Title |
LN229_DMSO CTRL_48hr_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
LN229
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: Vehicle control
|
| Treatment protocol |
Cells were seeded in 6-well plates and then incubated overnight to allow cells to attach. Cells were harvested after the treatment of 10 microM Momordicine or DMSO control for 48 hrs.
|
| Growth protocol |
Both cells were grown in DMEM medium with 2% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol according to manufacturer's protocol.
|
| Label |
Cy5
|
| Label protocol |
The 1 microg of total RNA was reverse-transcribed and amplified using MessageAmpTM aRNA Amplification Kit (Ambion). Indirect labeling the aa-UTP whereon by NHS-CyDye (Cy5, Amershan).
|
| |
|
| Hybridization protocol |
HOA v7.1 arrays were pre-heat at 60℃ for 10 mins, rehydrated by 100% ethanol following with deionized water. The slides were pre-hybridized with 5x SSPE, 0.1% SDS and 1% BSA at 42℃ for 2 hour. After the pre-hybridization, 5 microg Cy5-labeled aRNA was hybridize on HOA in the presentation of the Phalanx OneArray hybridization buffer in duplication.
|
| Scan protocol |
The arrays were scanned by Agilent G2505C scanner (635nm) and quantify the fluoresence intensity.
|
| Data processing |
Data processing was performed and calculated by limma package (Bioconductor). The control probes were filtered out and then normalized by the median scaling method between technical replicates. Pair-wise comparisons between samples were calculated by empirical bayes statistics and presented by log2 ratio and p-value.
|
| |
|
| Submission date |
May 03, 2023 |
| Last update date |
May 01, 2024 |
| Contact name |
Yung Lung Chang |
| E-mail(s) |
ylchang@mail.ndmctsgh.edu.tw
|
| Organization name |
National Defense Medical Center
|
| Department |
Biochemistry
|
| Street address |
Rm. 7309, 7F., No.161, Sec. 6, Minquan E. Rd., Neihu Dist.
|
| City |
Taipei |
| ZIP/Postal code |
114 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL21282 |
| Series (1) |
| GSE231550 |
Momordicine I Suppresses Glioma Growth by Promoting Apoptosis and Impairing Mitochondrial Oxidative Phosphorylation |
|
