|
| Status |
Public on Jan 17, 2024 |
| Title |
JC21_G007LK |
| Sample type |
RNA |
| |
|
| Source name |
Human Colorectal Cancer
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: JC21
treatment: 0.3 uM G007LK for 24 h
|
| Treatment protocol |
The patient-derived CRC cells, JC21 and JC475, were left untreated (DMSO) or treated with 0.3 µM G007LK or RK582 for 24 h.
|
| Growth protocol |
JC21 and JC475 cells were maintained in in the StemPro ESC medium (Life technologies) supplemented with 10 μM Y-27632 in a humidified atmosphere of 5% CO2 and 95% air at 37 °C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using Fast Gene RNA Basic kit (Nippon Genetics) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 100ng total RNA.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Data processing |
Gene-level expression signals were obtained by processing of raw data with the MAS5 procedure using GeneSpring GX (Agilent Technologies).
|
| |
|
| Submission date |
May 10, 2023 |
| Last update date |
Jan 17, 2024 |
| Contact name |
Tetsuo Mashima |
| E-mail(s) |
ttmjmj@gmail.com
|
| Phone |
81-3-3520-0111
|
| Organization name |
Japanese Foundation for Cancer Research
|
| Department |
Cancer Chemotherapy Center
|
| Lab |
Division of Molecular Biotherapy
|
| Street address |
Koto-ku, Ariake 3-8-31
|
| City |
Tokyo |
| ZIP/Postal code |
135-8550 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE232209 |
Gene expression data of APC-mutated colorectal cancer patient-derived cells treated with tankyrase inhibitors |
|
