|
| Status |
Public on Jun 09, 2016 |
| Title |
ovarian cancer cell line SKOV-3 rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
ovarian cancer cell line SK-OV-3 passage 5
|
| Organism |
Homo sapiens |
| Characteristics |
species: human
gender: female
cell type: ovarian epithelial
cell line: SKOV-3
p53 status: null
treatment: none
pathology: adenocarcinoma
cell passage: P5
comments: moderately resistant to cisplatin
sample: control 1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ovarian cancer cell lines were grown in culture. TRI reagent was added to the cells for harvesting. Bromochloropropane and sodium acetate were added to the samples and centrifuged. The RNA-containing upper layer was removed and the RNA purified on an RNeasy extraction column (Qiagen). The sample was treated with an on-column DNase treatment (RNase-free DNase, Qiagen). The purity and quantity of total RNA was evaluated by spectrophotometry and agarose gel electrophoresis. (Eyster and Brannian, Meth Molec Biol 2010;590:71-89)
|
| Label |
Alexa-647
|
| Label protocol |
Biotinylated cRNA was prepared using the MessageAmp II-Biotin Enhanced Kit from Ambion from 1.0 microgram total RNA starting material. (Eyster and Brannian, Meth Molec Biol 2010;590:71-89)
|
| |
|
| Hybridization protocol |
10 micrograms of biotinylated cRNA were mixed with fragmentation buffer and heated to 94C for 20 min. The fragmented cRNA was mixed with CodeLink hybridization buffer, loaded on the microarray slides, and hybridized for 18 hours at 37C. (Eyster and Brannian, Meth Molec Biol 2010;590:71-89)
|
| Scan protocol |
The slides were scanned in an Axon GenePix 4000B scanner.
|
| Description |
SK-OV-3 cell line was obtained from American Type Culture Collection.
|
| Data processing |
global median normalization, CodeLink Expression Analysis 5.0
|
| |
|
| Submission date |
Jun 06, 2011 |
| Last update date |
Jun 09, 2016 |
| Contact name |
Kathleen M Eyster |
| E-mail(s) |
Kathleen.Eyster@usd.edu
|
| Organization name |
University of South Dakota
|
| Department |
Basic Biomedical Sciences
|
| Street address |
414 E. Clark St.
|
| City |
Vermillion |
| State/province |
SD |
| ZIP/Postal code |
57069 |
| Country |
USA |
| |
|
| Platform ID |
GPL2895 |
| Series (1) |
| GSE29763 |
Gene expression profile associated with a cytostatic does of mifepristone in ovarian cancer cells by microarray analysis |
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