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Sample GSM738236 Query DataSets for GSM738236
Status Public on Dec 31, 2011
Title Whole Tissue_SD 4h Light_bhlh93_rep3
Sample type RNA
Source name Whole tissue, 10d old in SD
Organism Arabidopsis thaliana
Characteristics cultivar: bhlh93-1 mutant
time: 4 hr
Treatment protocol Samples were harvested in dark and after 4h light treatment in SD growth conditions.
Growth protocol Seeds were surface sterilized and plated on Murashige and Skoog growth medium (GM) containing 0.9% agar without Suc (GM - Suc) as described (Shen et al., 2005). After 4 d of stratification at 4oC, seeds were exposed to SD.
Extracted molecule total RNA
Extraction protocol For microarray, total RNA was isolated from 10d-old bhlh93-1 mutant and wild-type Col-0 seedlings grown under SD using the RNeasy plant mini kit (Qiagen). Five µg RNA from 10d old SD grown seedling of Wt and bhlh93-1 mutants was reverse transcribed using the RT-PCR kit from Invitrogen.
Label Cy3
Label protocol Double stranded cDNA was created using the Invitrogen double stranded-cDNA synthesis kit(Cat #11917010) according to Roche Nimblegen instructions (Roche protocol version 2.3) using an oligo-dT primer. Double-stranded cDNA was then labeled using Cy3-labeled nonamers (TriLink, San Diego, CA) and Klenow (large fragment, NEB cat #M0210M) also according to the Roche protocol version 2.3).
Hybridization protocol Microarray hybridization experiment was performed according to user manual of Roche Nimbelgen Arabidopsis thaliana 12x135K Array (090717 Athal TAIR9 exp HX12; Cat No. 05543746001). 4 micrograms of labeled product were resuspended in sample tracking control, applied to the arrays, and hybridized overnight in a Maui 4-position hybridization chamber, according to Roche Nimblegen protcol version 2.3. The arrays were then washed and dried according to the same protocol.
Scan protocol Arrays were scanned and analyzed according to Roche Nimblegen protcol version 2.3. Briefly, the arrays were scanned on an Axon 4000B scanner set at 5 micron resolution with 100% laser power and PMT gain between 500 and 600. The resulting TIF images were post-processed using Nimblescan version 2.3 to identify probe locations and each array was visually inspected for proper grid alignment.
Description 394517_A12_22Feb10_532.pair
Data processing The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
Submission date Jun 07, 2011
Last update date Dec 31, 2011
Contact name Enamul Huq
Phone 5124716658
Organization name University of Texas at Austin
Department MCDB
Lab Huq lab
Street address 24th St. and Whitis BIO labs ,
City Austin
State/province TX
ZIP/Postal code 78712
Country USA
Platform ID GPL13697
Series (1)
GSE29786 Role of bHLH93 in controlling flowering time in Arabidopsis

Data table header descriptions
VALUE Log scale gene expression values were calculated using a robust multiarray analysis (RMA).

Data table
AT1G01010_1 556.2540019
AT1G01020_1 472.0840729
AT1G01020_2 142.3698652
AT1G01030_1 159.5649529
AT1G01040_1 1025.989321
AT1G01046_1 86.87685666
AT1G01050_1 694.8221809
AT1G01060_1 2263.59478
AT1G01060_3 96.14233917
AT1G01070_1 241.6876593
AT1G01070_2 70.95340428
AT1G01073_1 99.25721016
AT1G01080_1 2205.20949
AT1G01080_2 551.5699311
AT1G01090_1 8588.556744
AT1G01100_1 1783.62919
AT1G01100_2 2455.498116
AT1G01100_3 2407.466487
AT1G01110_1 311.6752963
AT1G01110_2 1213.703241

Total number of rows: 37118

Table truncated, full table size 865 Kbytes.

Supplementary file Size Download File type/resource
GSM738236.calls.gz 342.9 Kb (ftp)(http) CALLS
GSM738236.pair.gz 2.2 Mb (ftp)(http) PAIR
Processed data included within Sample table
Processed data provided as supplementary file

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