|
| Status |
Public on Nov 20, 2023 |
| Title |
C57BL6/N_HSP27Overexpression Transgenic(TG)_Bleomcyin (1.25mg/kg) |
| Sample type |
RNA |
| |
|
| Source name |
Whole lung_14days_1.25mg/kg
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
strain: C57BL6/N
age: 6 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Amplified and labeled cRNA was purified on cRNA Cleanup Module (Agilent Technology) according to the manufacturer’s protocol. Labeled cRNA target was quantified using ND-1000 spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE).
|
| |
|
| Hybridization protocol |
After checking labeling efficiency, fragmentation of cRNA was performed by adding 10X blocking agent and 25X fragmentation buffer and incubating at 60oC for 30 min. The fragmented cRNA was resuspended with 2X hybridization buffer and directly pipetted onto assembled Agilent’s Canine Oligo Microarray (44K). The arrays hybridized at 65oC for 17 hours using Agilent Hybridization oven (Agilent Technology, USA). The hybridized microarrays were washed as the manufacturer’s washing protocol (Agilent Technology, USA).
|
| Scan protocol |
The hybridized images were scanned using Agilent’s DNA microarray scanner and quantified with Feature Extraction Software (Agilent Technology, Palo Alto, CA).
|
| Data processing |
Data was extracted using GeneSpringGX 7.3 (Agilent Technology, USA). Raw intensity data was globally normalized (Cheadle et al.(2003).
|
| |
|
| Submission date |
May 30, 2023 |
| Last update date |
Nov 20, 2023 |
| Contact name |
Yun-Sil Lee |
| E-mail(s) |
yslee0425@ewha.ac.kr
|
| Organization name |
Ewha Womans University
|
| Department |
College of Pharmacy
|
| Street address |
52, Ewhayeodae-gil, Seodaemun-gu
|
| City |
Seoul |
| ZIP/Postal code |
03760 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE233735 |
HSP27 (Heat Shock Protein 27, HSPB1) overexpression effect on bleomycin-induced PF (Pulmonary fibrosis) mice model |
|
