|
| Status |
Public on Feb 27, 2024 |
| Title |
B6; 129P2-Pde2A < tm1Dgen> KO_D |
| Sample type |
SRA |
| |
|
| Source name |
embryonic heart
|
| Organism |
Mus musculus |
| Characteristics |
tissue: embryonic heart
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from embryonic cardiac tissue using Total RNA Purification kit (Norgen). RNA-sequencing, the integrity and purity of the RNA was initially checked at the Bioanalyzer (Agilent 2100 bioanalyzer) by using RNA 6000 Nano Agilent kit
stranded mRNA-seq libraries were prepared with the NEBNext RNA Ultra II kit with 300 ng as an input as 12 PCR cycles
prepared libaries were pooled in the equimolar amount and sequenced with a single-end running mode with the Illumina NextSeq 500 instrument
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Illumina data processing pipeline
STAR aligner
Assembly: GRCm38-mm10
Supplementary files format and content: fiiles ".txt.gz" contain generated sequences in fasta format, files ".bam" contain processed sequences aligned to a reference genome
Supplementary files format and content: files ".out.tab" contain processed read counts per gene
|
| |
|
| Submission date |
Jun 07, 2023 |
| Last update date |
Feb 27, 2024 |
| Contact name |
Manuela Pellegrini |
| E-mail(s) |
manuela.pellegrini@cnr.it
|
| Organization name |
IBBC-CNR
|
| Street address |
Via E Ramarini
|
| City |
Monterotondo |
| State/province |
Rome |
| ZIP/Postal code |
00015 |
| Country |
Italy |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE234392 |
Effect of cAMP signaling activation during heart development in Pde2A deficient embryos. |
|
| Relations |
| BioSample |
SAMN35668496 |
| SRA |
SRX20634810 |
