|
| Status |
Public on Apr 08, 2012 |
| Title |
Bone biopsy_pre_3 |
| Sample type |
RNA |
| |
|
| Source name |
Bone biposy pre
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
age: 30
adenoma: pituitary
tissue: bone
biopsy collection: pre-surgery
time pre_post surgery (years): 0.15
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction followed by Rneasy clean up kit (qiagen) was performed according to the manufacturer´s instruction
|
| Label |
biotin
|
| Label protocol |
Double-stranded cDNA and biotin-labeled cRNA probes were made from 5 μg of total RNA by use of GeneChip® Expression 3' Amplification One-Cycle Target Labeling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
The cRNA was hybridized to HG U133 plus 2.0 Arrays (Affymetrix) followed by washing and staining on the GeneChips Fluidics Station 450 (Affymetrix)
|
| Scan protocol |
Signal intensities were detected by Affymetrix GeneChip Scanner 3000
|
| Description |
LJB Pre 21_1.CEL
|
| Data processing |
The scanned images were processed using GCOS 1.4 (Affymetrix) according to the manufacturer. The resulting data sets (CEL files) were imported into ArrayAssist Expression Software (v5.2.0;Iobion Informatics LLC) and normalized using the PLIER algorithm in Array Assist to calculate relative signal values for each probe set.
|
| |
|
| Submission date |
Jun 22, 2011 |
| Last update date |
Apr 08, 2012 |
| Contact name |
Tove Lekva |
| E-mail(s) |
tove.lekva@medisin.uio.no, tove.lekva@ous-hf.no
|
| Organization name |
OUS-HF, Rikshospitalet
|
| Department |
Section of Specialized Endocrinology and Institute for Internal Medicine
|
| Street address |
Sognsvannsveien 20
|
| City |
Oslo |
| ZIP/Postal code |
0027 |
| Country |
Norway |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE30159 |
Gene expression analysis of bone biospies from nine patients with endogenous Cushings syndrome before and after treatment |
|
