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Sample GSM746848 Query DataSets for GSM746848
Status Public on Apr 08, 2012
Title Bone biopsy_post_3
Sample type RNA
 
Source name Bone biopsy post
Organism Homo sapiens
Characteristics gender: female
age: 31
adenoma: pituitary
tissue: bone
biopsy collection: post-surgery
time pre_post surgery (years): 0.15
Extracted molecule total RNA
Extraction protocol Trizol extraction followed by Rneasy clean up kit (qiagen) was performed according to the manufacturer´s instruction
Label biotin
Label protocol Double-stranded cDNA and biotin-labeled cRNA probes were made from 5 μg of total RNA by use of GeneChip® Expression 3' Amplification One-Cycle Target Labeling kit (Affymetrix).
 
Hybridization protocol The cRNA was hybridized to HG U133 plus 2.0 Arrays (Affymetrix) followed by washing and staining on the GeneChips Fluidics Station 450 (Affymetrix)
Scan protocol Signal intensities were detected by Affymetrix GeneChip Scanner 3000
Description LJB Post 22_1.CEL
Data processing The scanned images were processed using GCOS 1.4 (Affymetrix) according to the manufacturer. The resulting data sets (CEL files) were imported into ArrayAssist Expression Software (v5.2.0;Iobion Informatics LLC) and normalized using the PLIER algorithm in Array Assist to calculate relative signal values for each probe set.
 
Submission date Jun 22, 2011
Last update date Apr 08, 2012
Contact name Tove Lekva
E-mail(s) tove.lekva@medisin.uio.no, tove.lekva@ous-hf.no
Organization name OUS-HF, Rikshospitalet
Department Section of Specialized Endocrinology and Institute for Internal Medicine
Street address Sognsvannsveien 20
City Oslo
ZIP/Postal code 0027
Country Norway
 
Platform ID GPL570
Series (1)
GSE30159 Gene expression analysis of bone biospies from nine patients with endogenous Cushings syndrome before and after treatment

Data table header descriptions
ID_REF
VALUE Log2 PLIER signals

Data table
ID_REF VALUE
1007_s_at 7.88044
1053_at 7.3009
117_at 6.72327
121_at 7.70374
1255_g_at 3.5609
1294_at 8.2861
1316_at 5.73583
1320_at 5.38999
1405_i_at 8.10017
1431_at 4.0181
1438_at 5.32217
1487_at 7.74076
1494_f_at 5.83229
1552256_a_at 7.14831
1552257_a_at 7.69336
1552258_at 5.25393
1552261_at 5.23002
1552263_at 7.41062
1552264_a_at 9.36511
1552266_at 3.91747

Total number of rows: 54675

Table truncated, full table size 998 Kbytes.




Supplementary file Size Download File type/resource
GSM746848_LJB_Post_22_1.CEL.gz 5.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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