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Sample GSM7474563 Query DataSets for GSM7474563
Status Public on May 01, 2024
Title DT 36h
Sample type RNA
Source name Diphtheria-Toxin treated+ 36h recovery
Organism Mus musculus
Characteristics genotype: Atp4b-DTR
tissue: Gastric Corpus Tissue
Treatment protocol Diphtheria toxin (225ng/mouse) or saline vehicle was administered via ip injuection daily for three days. Mice were euthanized at 24, 36, 48, or 72 hours after final siphtheria toxin injection
Growth protocol Mice were grown in a temperature and light controlled mouse facility; littermate controls were used when possible. Adult mice age 8-12 weeks were used.
Extracted molecule total RNA
Extraction protocol Total RNA extracted, purified by RNEasy Micro Kit, and RNA intergrity verified on Agilent Bioanalyzer
Label Biotin
Label protocol Total RNA was extracted and used to prepare biotinylated cDNA with Affymetrix WT plus reagent kit
Hybridization protocol Labelled cDNA hybridized to Affymetrix GeneChip Mouse Gene 2.0 ST Arrays according to manufacturers instructions and placed in GeneChip Hybridization Oven 620 for 18hr at 45C. Microarrays were washes and stained with Affymettrix Fluidics Station 450.
Scan protocol Arrays were scanned with a GeneChip® 7G Scanner 3000 laser confocal slide scanner
Data processing Microarray data data expression was process using Command Console (Affymetrix, Inc) and the raw .CEL files were analyzed using Partek Genomic Suite v7.0 (Partek Inc., St Louis, MO). Microarray CEL files were imported into Partek and normalized using RMA.
Submission date Jun 12, 2023
Last update date May 01, 2024
Contact name Jason C Mills
Phone 713-798-4882
Organization name Baylor College of Medicine
Street address 1 Baylor Plaza
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
Platform ID GPL16570
Series (1)
GSE234777 Microarray analysis of regenerating parietal cells (PCs) in murine gastric epithelium recovering from Diphtheria toxin injury

Data table header descriptions
VALUE Quantification

Data table
17200001 5.19389
17200003 5.37739
17200005 2.95485
17200007 5.14177
17200009 5.25245
17200011 3.18238
17200013 4.47002
17200015 4.92933
17200017 2.92404
17200019 3.38141
17200021 4.57104
17200023 6.19468
17200025 4.49228
17200027 5.29771
17200029 3.73364
17200031 4.46264
17200033 4.22184
17200035 3.11982
17200037 4.53374
17200039 2.13157

Total number of rows: 41345

Table truncated, full table size 681 Kbytes.

Supplementary file Size Download File type/resource
GSM7474563_DT_36h.CEL.gz 8.3 Mb (ftp)(http) CEL
GSM7474563_DT_36h.chp.gz 250.8 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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