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Sample GSM7474565 Query DataSets for GSM7474565
Status Public on May 01, 2024
Title DT 48h rep2
Sample type RNA
Source name Diphtheria-Toxin treated+ 48h recovery
Organism Mus musculus
Characteristics genotype: Atp4b-DTR
tissue: Gastric Corpus Tissue
Treatment protocol Diphtheria toxin (225ng/mouse) or saline vehicle was administered via ip injuection daily for three days. Mice were euthanized at 24, 36, 48, or 72 hours after final siphtheria toxin injection
Growth protocol Mice were grown in a temperature and light controlled mouse facility; littermate controls were used when possible. Adult mice age 8-12 weeks were used.
Extracted molecule total RNA
Extraction protocol Total RNA extracted, purified by RNEasy Micro Kit, and RNA intergrity verified on Agilent Bioanalyzer
Label Biotin
Label protocol Total RNA was extracted and used to prepare biotinylated cDNA with Affymetrix WT plus reagent kit
Hybridization protocol Labelled cDNA hybridized to Affymetrix GeneChip Mouse Gene 2.0 ST Arrays according to manufacturers instructions and placed in GeneChip Hybridization Oven 620 for 18hr at 45C. Microarrays were washes and stained with Affymettrix Fluidics Station 450.
Scan protocol Arrays were scanned with a GeneChip® 7G Scanner 3000 laser confocal slide scanner
Data processing Microarray data data expression was process using Command Console (Affymetrix, Inc) and the raw .CEL files were analyzed using Partek Genomic Suite v7.0 (Partek Inc., St Louis, MO). Microarray CEL files were imported into Partek and normalized using RMA.
Submission date Jun 12, 2023
Last update date May 01, 2024
Contact name Jason C Mills
Phone 713-798-4882
Organization name Baylor College of Medicine
Street address 1 Baylor Plaza
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
Platform ID GPL16570
Series (1)
GSE234777 Microarray analysis of regenerating parietal cells (PCs) in murine gastric epithelium recovering from Diphtheria toxin injury

Data table header descriptions
VALUE Quantification

Data table
17200001 5.71106
17200003 4.87118
17200005 3.06803
17200007 4.57128
17200009 5.7013
17200011 3.22599
17200013 4.14731
17200015 5.2635
17200017 2.90492
17200019 3.32671
17200021 3.68395
17200023 6.09011
17200025 4.78198
17200027 5.10155
17200029 3.78474
17200031 4.0791
17200033 3.1816
17200035 3.30592
17200037 5.42659
17200039 2.55824

Total number of rows: 41345

Table truncated, full table size 681 Kbytes.

Supplementary file Size Download File type/resource
GSM7474565_DT_48h_B.CEL.gz 8.7 Mb (ftp)(http) CEL
GSM7474565_DT_48h_B.chp.gz 251.1 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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