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Sample GSM7474566 Query DataSets for GSM7474566
Status Public on May 01, 2024
Title DT 72h A rep1
Sample type RNA
Source name Diphtheria-Toxin treated+ 72h recovery
Organism Mus musculus
Characteristics genotype: Atp4b-DTR
tissue: Gastric Corpus Tissue
Treatment protocol Diphtheria toxin (225ng/mouse) or saline vehicle was administered via ip injuection daily for three days. Mice were euthanized at 24, 36, 48, or 72 hours after final siphtheria toxin injection
Growth protocol Mice were grown in a temperature and light controlled mouse facility; littermate controls were used when possible. Adult mice age 8-12 weeks were used.
Extracted molecule total RNA
Extraction protocol Total RNA extracted, purified by RNEasy Micro Kit, and RNA intergrity verified on Agilent Bioanalyzer
Label Biotin
Label protocol Total RNA was extracted and used to prepare biotinylated cDNA with Affymetrix WT plus reagent kit
Hybridization protocol Labelled cDNA hybridized to Affymetrix GeneChip Mouse Gene 2.0 ST Arrays according to manufacturers instructions and placed in GeneChip Hybridization Oven 620 for 18hr at 45C. Microarrays were washes and stained with Affymettrix Fluidics Station 450.
Scan protocol Arrays were scanned with a GeneChip® 7G Scanner 3000 laser confocal slide scanner
Data processing Microarray data data expression was process using Command Console (Affymetrix, Inc) and the raw .CEL files were analyzed using Partek Genomic Suite v7.0 (Partek Inc., St Louis, MO). Microarray CEL files were imported into Partek and normalized using RMA.
Submission date Jun 12, 2023
Last update date May 01, 2024
Contact name Jason C Mills
Phone 713-798-4882
Organization name Baylor College of Medicine
Street address 1 Baylor Plaza
City Houston
State/province Texas
ZIP/Postal code 77030
Country USA
Platform ID GPL16570
Series (1)
GSE234777 Microarray analysis of regenerating parietal cells (PCs) in murine gastric epithelium recovering from Diphtheria toxin injury

Data table header descriptions
VALUE Quantification

Data table
17200001 5.52157
17200003 5.21776
17200005 2.52307
17200007 4.03396
17200009 5.08079
17200011 3.75369
17200013 4.90947
17200015 5.13588
17200017 1.9903
17200019 3.70116
17200021 3.03888
17200023 5.53463
17200025 4.68007
17200027 5.13704
17200029 3.42171
17200031 3.93388
17200033 2.89096
17200035 3.74742
17200037 4.69905
17200039 2.58986

Total number of rows: 41345

Table truncated, full table size 682 Kbytes.

Supplementary file Size Download File type/resource
GSM7474566_DT_72h_A.CEL.gz 8.5 Mb (ftp)(http) CEL
GSM7474566_DT_72h_A.chp.gz 250.9 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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