|
| Status |
Public on Jul 01, 2011 |
| Title |
MOLM-14 DZNep, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
MOLM-14 DZNep treated
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MOLM-14
disease type: acute myeloid leukemia (AML)
|
| Treatment protocol |
DMSO or DZNep
|
| Growth protocol |
90% RPMI1640 + 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
200ng of total RNA were amplified and labeled using Affymetrix Whoe Transcript Sense Target Labeling and control reagents kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Biotinylated samples were hybridized onto the array for 16hrs and 60rpm in a GeneChip Hybridization Oven 640 (Affymetrix)
|
| Scan protocol |
GeneChips were scanned using the affymetrix scanner 3000 7G
|
| Description |
MOLM-14 cells were treated with DZNep
|
| Data processing |
The data were analyzed with Genespring GX. Raw signals were converted to expression value using RMA algorithm.
|
| |
|
| Submission date |
Jun 30, 2011 |
| Last update date |
Jul 01, 2011 |
| Contact name |
Jianbiao Zhou |
| E-mail(s) |
jianbiaozhou@yahoo.com
|
| Organization name |
Cancer Science Institute Singapore
|
| Street address |
28 Medical Drive
|
| City |
Singapore |
| ZIP/Postal code |
117456 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE30315 |
DMSO control and DZNep treated MOLM-14 cells |
|
