|
| Status |
Public on Jul 31, 2024 |
| Title |
heart_sleep fragmented_5 weeks_Control 2 |
| Sample type |
RNA |
| |
|
| Source name |
heart, sleep fragmented, 5 weeks, Control 2
|
| Organism |
Mus musculus |
| Characteristics |
tissue: heart
gender: female
age: 21 weeks
|
| Treatment protocol |
The ventricles were isolated from mice after intervention.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the heart using Total RNA Extraction Reagent (Invitrogen, Bio-Tek, Inc., USA). The RNA was further purified using RNeasy spin columns (Qiagen p/n 74104) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
The RNA was amplified and labeled using the Agilent Quick Amp One-Color labeling kit (Agilent p/n 5190-0442).
|
| |
|
| Hybridization protocol |
Then the RNA was hybridized using an Agilent Gene Expression Hybridization Kit (Agilent p/n 5188-5242).
|
| Scan protocol |
Following microarray hybridization and washing, the processed slides were scanned with an Agilent DNA microarray scanner (Agilent p/n G2565BA).
|
| Description |
gene expression after 5 weeks of sleep fragmentation
|
| Data processing |
The raw gene expression data were extracted from Agilent Feature Extraction Software (Version 10.5.1.1) and imported into Agilent GeneSpring GX software (version 11.0) for further analysis. The microarray data sets were normalized in GeneSpring GX using the Agilent Feature Extraction one-color scenario (primarily using quantile normalization).
|
| |
|
| Submission date |
Jul 03, 2023 |
| Last update date |
Jul 31, 2024 |
| Contact name |
Ling Zhong |
| E-mail(s) |
zhongling1222@gmail.com
|
| Organization name |
Peking Union Medical College Hospital
|
| Department |
Department of Endocrinology
|
| Street address |
Wangfujing Street
|
| City |
Beijing |
| ZIP/Postal code |
100730 |
| Country |
China |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE236358 |
Chronic sleep fragmentation reduces left ventricular contractile function and alters gene expression related to innate immune response and circadian rhythm in the mouse heart |
|
