genotype: C24 x Col-0 days after sowing: 10 date of crna synthesis: 2008-10-16 tissue: whole seedling
Treatment protocol
stable 16/8 hr photoperiod with 20°/19°C at 120 µmol m-2 s-1
Growth protocol
For early timepoints 4-6 days after sawing (DAS) seeds were sown onto nylon mesh (pore size 200 µm) covering 80 ml of a 1:1 mixture of GS90 soil (Gebrüder Patzer, Sinntal-Jossa, Germany) and vermiculite (Deutsche Vermiculite Dämmstoff, Sprockhövel, Germany) moistened with 40 ml tap water in 15 cm Petri dishes. Each Petri dish was divided in four equal-sized quarters, marked with pencil on the nylon mesh. Approximately 50 seeds of the four tested genotypes Col-0, C24, Col-0xC24-F1 and C24xCol-0-F1 were placed in one quarter each. The Petri dishes were sealed with micropore surgical tape (3M, Neuss, Germany). Seedlings were grown in four independent replicates in a controlled reach-in growth chamber under long-day conditions (16/8 hr photoperiod with 20°/19°C at 120 µmol m-2 s-1). For later time points (10, 15, 20, 25, and 30 DAS) five plants of each genotype were grown in pots (16 cm diameter) in a controlled walk-in phytotron under the same conditions as described above, in four independent replicates. Samples were harvested into liquid nitrogen in the middle of the light period between 11 am to 1 pm. This experiment was repeated four times and the RNA obtained from the four extractions per genotype and time point was pooled for the hybridization on Ath1- Affymetrix expression arrays.
genotype: C24 x Col-0 days after sowing: 15 date of crna synthesis: 2008-10-22 tissue: whole seedling
Treatment protocol
stable 16/8 hr photoperiod with 20°/19°C at 120 µmol m-2 s-1
Growth protocol
For early timepoints 4-6 days after sawing (DAS) seeds were sown onto nylon mesh (pore size 200 µm) covering 80 ml of a 1:1 mixture of GS90 soil (Gebrüder Patzer, Sinntal-Jossa, Germany) and vermiculite (Deutsche Vermiculite Dämmstoff, Sprockhövel, Germany) moistened with 40 ml tap water in 15 cm Petri dishes. Each Petri dish was divided in four equal-sized quarters, marked with pencil on the nylon mesh. Approximately 50 seeds of the four tested genotypes Col-0, C24, Col-0xC24-F1 and C24xCol-0-F1 were placed in one quarter each. The Petri dishes were sealed with micropore surgical tape (3M, Neuss, Germany). Seedlings were grown in four independent replicates in a controlled reach-in growth chamber under long-day conditions (16/8 hr photoperiod with 20°/19°C at 120 µmol m-2 s-1). For later time points (10, 15, 20, 25, and 30 DAS) five plants of each genotype were grown in pots (16 cm diameter) in a controlled walk-in phytotron under the same conditions as described above, in four independent replicates. Samples were harvested into liquid nitrogen in the middle of the light period between 11 am to 1 pm. This experiment was repeated four times and the RNA obtained from the four extractions per genotype and time point was pooled for the hybridization on Ath1- Affymetrix expression arrays.
