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Status |
Public on Dec 07, 2011 |
Title |
Nuclei_15min_(Nuc_20110428_4) |
Sample type |
SRA |
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Source name |
input DNA
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Organism |
Saccharomyces cerevisiae |
Characteristics |
strain: Sby2688 medium: YEPD grown:OD=0.8 antibody: none sample type: All MNase-protected DNA fragments, 15 minute digestion
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Extracted molecule |
genomic DNA |
Extraction protocol |
Yeast nuclei were prepared as described [Furuyama, S., and Biggins, S. (2007), Centromere identity is specified by a single centromeric nucleosome in budding yeast, Proc Natl Acad Sci USA 104, 14706-14711], flash-frozen in liquid nitrogen and stored at -80oC. Nuclei were thawed at room temperature and digested with MNase as described [Furuyama and Biggins, (2007)]. DNA was extracted using a standard protocol [Mito, Y., Henikoff, J., and Henikoff, S. (2005), Genome-scale profiling of histone H3.3 replacement patterns, Nat Genet 37, 1090-1097]. A modified Illumina Solexa library protocol was used as described in supplementary file Solexa_library_protocol_GEO.pdf.
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Library strategy |
MNase-Seq |
Library source |
genomic |
Library selection |
MNase |
Instrument model |
Illumina HiSeq 2000 |
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Description |
All MNase-protected DNA fragments, 15 minute digestion
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Data processing |
1. We used Novoalign to map paired-end reads to release 61 (UCSC sacCer2) of the S.cervisiae genomic sequence obtained from downloads.yeastgenome.org. 50bp reads were trimmed to 25bp. If a read was mapped to multiple locations, one location was picked at random. (Supplementary file Nuc_20110428_4..sam) 2. For each base pair in the genome, we counted the number of paired-end fragments aligned over it. 3. We normalized base pair counts by dividing by the total number of counts for all base pairs and then multiplying by the total number of base pairs in the genome. (Supplementary file Nuc_20110428_4..wig) 4. We broke down aligned paired-end fragments into sub-groups by insert size length and repeated steps 2. and 3. for the paired-end fragments in each sub-group.
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Submission date |
Jul 05, 2011 |
Last update date |
Jun 11, 2013 |
Contact name |
Jorja Henikoff |
E-mail(s) |
jorja@fhcrc.org
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Phone |
206-667-4850
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Organization name |
Fred Hutchinson Cancer Research Center
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Department |
Basic Sciences
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Lab |
Henikoff
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Street address |
1100 Fairview AV N, A1-162
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City |
Seattle |
State/province |
WA |
ZIP/Postal code |
98109-1024 |
Country |
USA |
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Platform ID |
GPL13821 |
Series (1) |
GSE28298 |
Tripartite organization of centromeric chromatin in budding yeast |
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Relations |
BioSample |
SAMN02198202 |
Supplementary file |
Size |
Download |
File type/resource |
GSM754366.wig.gz |
44.7 Mb |
(ftp)(http) |
WIG |
GSM754366_Nuc_20110428_4.sam.gz |
616.0 Mb |
(ftp)(http) |
SAM |
Processed data provided as supplementary file |
Raw data not provided for this record |
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