|
| Status |
Public on Jul 23, 2011 |
| Title |
DFC H3K4me3 Hs35b_P06R |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
ChIP DNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: differentiated hESC
chip antibody: H3K4me3
cell line: H1
chip antibody manufacturer: Upstate
chip antibody catalog #: 07-473
|
| Treatment protocol |
H1 hESCs were grown on matrigel in mTeSR1 supplemented with 200ng/mL BMP4 for 5 days
|
| Growth protocol |
H1 hESCs were grown on matrigel in mTeSR1
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatin extraction, sonication, ChIP and LM-PCR were performed following previously published protocols from the Ren Lab (Li et al., 2005; Heintzman et al., 2007 & 2009).
|
| Label |
biotin
|
| Label protocol |
All samples were labeled, hyb'd and scanned by the GeneChip Microarray Core at UCSD following Affymetrix specifications.
|
| |
|
| Channel 2 |
| Source name |
input DNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: hESC
chip antibody: none
cell line: H1
|
| Treatment protocol |
H1 hESCs were grown on matrigel in mTeSR1 supplemented with 200ng/mL BMP4 for 5 days
|
| Growth protocol |
H1 hESCs were grown on matrigel in mTeSR1
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatin extraction, sonication, ChIP and LM-PCR were performed following previously published protocols from the Ren Lab (Li et al., 2005; Heintzman et al., 2007 & 2009).
|
| Label |
biotin
|
| Label protocol |
All samples were labeled, hyb'd and scanned by the GeneChip Microarray Core at UCSD following Affymetrix specifications.
|
| |
|
| |
| Hybridization protocol |
All samples were labeled, hyb'd and scanned by the GeneChip Microarray Core at UCSD following Affymetrix specifications.
|
| Scan protocol |
All samples were labeled, hyb'd and scanned by the GeneChip Microarray Core at UCSD following Affymetrix specifications.
|
| Description |
H3K4me3 ChIP on H1 hESCs treated w. BMP4 for 5d
|
| Data processing |
data aligned to hg17
|
| |
|
| Submission date |
Jul 06, 2011 |
| Last update date |
Jul 28, 2011 |
| Contact name |
Gary Chung Hon |
| Organization name |
UT Southwestern
|
| Department |
OB/GYN
|
| Street address |
5323 Harry Hines Blvd.
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL4915 |
| Series (1) |
| GSE30434 |
ChIP-chip for histone modifications in hESCs and differentiated hESCs |
|
