|
| Status |
Public on Jul 06, 2023 |
| Title |
genomic DNA from Blood from ShamOldIso4 |
| Sample type |
genomic |
| |
|
| Source name |
Blood from ShamOldIso4
|
| Organism |
Mus musculus |
| Characteristics |
age (years): 1.75
mouse strain: C57BL/6 WT
Sex: Female
mouse id: ShamOldIso4
tissue: Blood
|
| Treatment protocol |
Female C57Bl/6 mice were pre-screened to minimize body size differences, and were randomly assigned to pairs. Isochronic pairs consisted of two 3-month-old or 20-month-old mice and heterochronic pairs consisted of one 3-month-old mouse and one 20-month-old mouse. Both mice were anesthetized by isoflurane and opposing lateral sides of the abdomen were sutured from elbow to knee (the same distance as standard parabiosis) to create a physical attachment between the two mice. Importantly, for the mock parabiosis, no incisions were made to the skin or fascia prior to attachment thus preventing the generation of shared vasculature. We confirmed separation of circulation by testing the blood glucose response in both parabionts to an IP glucose bolus delivered to one of the partners. All mock parabiosis pairs were attached for 5 weeks.
|
| Growth protocol |
This experiment was approved by the Duke University IACUC. C57Bl/6 mice were obtained from Jackson Laboratories and acclimated to our animal facility for at least 48 h before being subjected to any experimental manipulation. Aged C57Bl/6 mice for parabiosis experiments were obtained from the NIA aged rodent colony. Mice were maintained in a barrier facility in sterilized, ventilated cages and fed standard laboratory chow (LabDiet 5053) and reverse osmosis drinking water ad libitum and maintained on a 12h:12h light:dark cycle. Mice were generally housed socially. Mice were humanely euthanized at the conclusion of each experiment by CO2 exposure followed by cervical dislocation.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was isolated from whole blood using the DNeasy Blood and Tissue Kit (Qiagen) following the manufacturer’s protocol.
|
| Label |
Cy3,Cy5
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
Standard Illumina protocol
|
| Description |
Old isochronic sham parabiosis
206604260011_R01C02
|
| Data processing |
For mammalian microarray analysis, raw methylation data were first normalized and beta values were calculated using the SeSAMe R package.
|
| |
|
| Submission date |
Jul 05, 2023 |
| Last update date |
Jul 06, 2023 |
| Contact name |
Jesse Poganik |
| Organization name |
Brigham and Women's Hospital, Harvard Medical School
|
| Department |
Medicine/Genetics
|
| Lab |
Gladyshev Lab
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL32543 |
| Series (2) |
| GSE224447 |
DNA methylation and gene expression changes in mice induced by parabiosis and recovery |
| GSE236617 |
DNA methylation changes in mice induced by mock parabiosis |
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